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01 February 2020 5,449 3 View

Why did I get negative peaks in HPLC with a UV detector and a SEC column?

Hello, Today, when I analyzed my nectar sample (containing plant secondary metabolites, amino acids and proteins) using a HPLC with a UV detector (220nm) and a SEC column, NaOAC buffer as mobile...

11 December 2019 6,709 9 View

How to isolate peptides from sugar solution?

Dear all, I try to isolate peptide (MW less than 3000Da ) from sugar solution (sugar concentration ~30%). The concentration of the peptide might be very low, but it is ok if the isolated peptides...

31 December 2018 4,261 3 View

What is the white grains on the leaves, wax secretion?

Hello, I saw many white grains on the leaves of my Hibiscus plants in the green house. Does any people know what is it? It doesn't look like any insects' egg. And it can't dissolve in water. Wax? Zha

08 September 2018 2,532 0 View

Is it possible that L-gulonolactone oxidase diminish hydrogen peroxide by using ascorbate and produce L-gulono-1,4-lactone and oxygen?

Dear Colleagues We know that L-gulonolactone oxidase catalyzes the reaction of L-gulono-1,4-lactone with oxygen to L-xylo-hex-3-gulonolactone and hydrogen peroxide. Is this reaction reversible? Or...

04 May 2017 3,537 0 View

How to purify FAD-dependent enzyme?

Dear Colleagues I am trying to purify a FAD-binding protein. Is there a reliable method I can use to purity such proteins? Best Zha

03 April 2017 5,107 9 View

How to quantify FAD in biological samples?

Dear Colleagues, I am looking for a method that could be used to measure FAD concentration (Flavin adenine dinucleotide) in biological samples, such as nectar or plant bleeding sap. Hope any...

02 March 2017 5,016 2 View

Why are monocots and dicots mixed in the phylogeny tree?

04 May 2016 3,045 10 View

Please help to identify this plant.

Please help to identify this plant. I found it in a green house. A species from Liliaceae, but not sure about the exact name. Please help to identify it.

03 April 2016 3,333 10 View

How many times can Tenax TA column be used for floral scent analysis?

10 November 2014 2,900 3 View

What are the countries most affected by extreme rainfall as a result of climate change?

Increased heat, drought and insect outbreaks, all associated to climate change, have increased wildfires. Decreasing water supplies, declined agricultural yields, health impacts in cities owing to...

01 March 2021 7,168 9 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

The Shannon's index is inversely proportional to Simpson's index (1-D)?

I'm having a problem with my bloxplot with Shannon's and Simpson's indexs. The value of Shannon for my Community 1 indicating higher diversity (in relation of the Community 2), but have lower...

26 February 2021 3,033 9 View

Please help in identification of this insect?

This insect is minute in size around 1 mm and not clearly visible with naked eye.

22 February 2021 5,907 11 View

Purification of proteins from baculovirus-infected insect cells?

18 February 2021 6,665 6 View

Any recommendations for methods to analyze elephant space-use (using GPS) and correlating this with changes in NDVI?

For my Masters I am looking into elephant space-use and its effect on vegetation change. I have elephant GPS collar data and was going to use Google Earth Engine to obtain NDVI values. I would...

16 February 2021 7,097 3 View

How to consider effects of rapid warm adaptation in modeling of ecological impacts of warming?

I would highly appreciate it if my fellow ecologists (biologists) provide their opinion on the thoughts below [esp., shortly tell us which path may be more effective, if they know another way, if...

15 February 2021 3,642 6 View

Extraction of DNA from insect samples conserved in silica gel?

Hi everybody, can anybody suggest how to extract DNA from insect samples conserved in silica gel (in my case, mosquitoes)? Does anybody know a commericial kit which does that or a particular...

10 February 2021 3,392 4 View

How do I prepare and store insect samples for JH measurements?

I need to preserve termite samples for future quantification of juvenile hormone in their bodies. Or extract the Juvenile Hormone and keep that samples stored for future quantification. I may need...

09 February 2021 1,995 1 View