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01 February 2020 5,449 3 View

Why did I get negative peaks in HPLC with a UV detector and a SEC column?

Hello, Today, when I analyzed my nectar sample (containing plant secondary metabolites, amino acids and proteins) using a HPLC with a UV detector (220nm) and a SEC column, NaOAC buffer as mobile...

11 December 2019 6,709 9 View

What is the white grains on the leaves, wax secretion?

Hello, I saw many white grains on the leaves of my Hibiscus plants in the green house. Does any people know what is it? It doesn't look like any insects' egg. And it can't dissolve in water. Wax? Zha

08 September 2018 2,532 0 View

Is it possible that L-gulonolactone oxidase diminish hydrogen peroxide by using ascorbate and produce L-gulono-1,4-lactone and oxygen?

Dear Colleagues We know that L-gulonolactone oxidase catalyzes the reaction of L-gulono-1,4-lactone with oxygen to L-xylo-hex-3-gulonolactone and hydrogen peroxide. Is this reaction reversible? Or...

04 May 2017 3,537 0 View

How to purify FAD-dependent enzyme?

Dear Colleagues I am trying to purify a FAD-binding protein. Is there a reliable method I can use to purity such proteins? Best Zha

03 April 2017 5,107 9 View

How to quantify FAD in biological samples?

Dear Colleagues, I am looking for a method that could be used to measure FAD concentration (Flavin adenine dinucleotide) in biological samples, such as nectar or plant bleeding sap. Hope any...

02 March 2017 5,016 2 View

Why are monocots and dicots mixed in the phylogeny tree?

04 May 2016 3,045 10 View

Please help to identify this plant.

Please help to identify this plant. I found it in a green house. A species from Liliaceae, but not sure about the exact name. Please help to identify it.

03 April 2016 3,333 10 View

How many times can Tenax TA column be used for floral scent analysis?

10 November 2014 2,900 3 View

Can someone help with identifying this beetle (see attached)?

Can anyone help with identifying this beetle? It is less than 0.5 cm long. The photo was taken in China.

08 September 2014 2,703 8 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

How to choose ANN number of hidden layers and nodes?

Hello, Could you please share any interesting research explaining how to choose the number of hidden layers and nodes per layer in case of regression problems using ANN? Thank you, any help would...

01 March 2021 6,200 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

How to improve and implement WBAN MAC and Radio modules under Castalia 3.2 ?

Hello researchers, Hello Castalia3.2 researchers I am a beginner with Castalia, I work on optimization of RFID systems used in wireless body networks. I simulated a WBAN with 10 nodes and one...

28 February 2021 400 1 View

How could I take into consideration the weights of observation in a system of seemingly unrelated regressions (SUR) in rstudio?

I build a model of 4 regression equations (probit and OLS) in R studio. I need to take the weights of observations into consideration. While it is simple to do so for every regression equation,...

27 February 2021 1,233 1 View