Why can AF488 and BV510 be used together?

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Isolation of naive mouse T cells, should I positively select on CD62L after I negatively select?

Does anti-CD62L antibody lead to some form of signaling on the naive T cells?

07 August 2018 2,879 2 View

Can I use anti-CD11c-PE and anti-TCRb-PE in the same staining panel since they are expressed on different cells?

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Protein Expression Level Reference Online for flow cytometry panel design?

Hello, I am looking for any online reference on any/all available protein expression data on any cell type to aid in the optimal design of flow cytometry panels. If anyone knows of any such...

01 February 2018 6,142 3 View

Setting voltage for compensation control vs actual samples?

I am newbie to flow cytometry. I use a LSR fortessa with FACSDiva software. When I run compensation controls, I have to set the voltages for each comp control to minimize the histogram overlaps...

11 December 2017 2,910 0 View

Do I need to run compensation controls for the same panel every time?

I am quite new to flow so I was just wandering if I have to do it every time. Seems I can use previous compensation setting.

10 November 2017 3,650 2 View

Not enough cells for FMO control, what's the alternative?

Is there an alternative to FMO controls if you don't have enough cells for FMO controls?

10 November 2017 8,659 3 View

I see "free acid" on HEPES bottle on some brands but not others, what does it mean?

see question

09 October 2017 606 0 View

Do FMO controls need same number of cells as actual samples?

I am flowing a lot of cells per sample

09 October 2017 2,518 3 View

What is the best digestive enzyme solution to digest mouse thymus for single cell suspension?

I want to look at dendritic cells by flow cytometry. I've been using Collagenase IV plus DNase I in blank RPMI1640 media.

06 July 2017 7,246 2 View

Does anyone have experience doing IHC for FFPE mouse ear? How do you keep the tissue from falling apart?

The blocks were cut into 6um sections and let float in 43C water bath before scooping up with microscope slide (time in water approx

31 December 2015 2,847 4 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

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How to make a Lateral flow control line more uniform?

We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...

31 July 2024 4,510 3 View

Suggestions for the Annexin V Positive Control ?

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29 July 2024 8,624 1 View

Why cannot i find my protein on cell surface after antibiotic selection of expressing plasmid?

I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...

28 July 2024 3,178 3 View

Problem with Two-phase simulation?

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25 July 2024 2,309 5 View

Flow through curved domains?

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BD Cell Viability Kit - TO evaporation?

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Can I use CCCP instead of H₂O₂ as a positive control for ROS measurement through flow cytometry?

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I came across oscillations in a pressure profile for a pipeline flow along the axis of a cylinder, how do I prove that these are not numerical err?

In terms of CFD, we often analyze the stability of the error using Von-Neumann analysis, especially for FDM based problems. Should we follow the same approach for a compressible fluid flow using FVM ?

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Qingda Meng

Depend your filter. The overlaps can be removed after compensation.

Please learn more about compensation.

Jan Martinek

These two colors are exited by different lasers (UV laser for BV colors and "green" laser for AF488)

Yong Lin

Jan Martinek Is it because of time delay from different lasers that the software can separate those out?

Kerstin Andersson

You can use them together as long as your flow cytometer have separated laser beams. After that it's a matter of compensation to get it right.

Kerstin and Jan is right, if you have separated laser beams, it will be good.

Ann Atzberger

Different laser beams, different optical pathways.