I am newbie to flow cytometry.
I use a LSR fortessa with FACSDiva software.
When I run compensation controls, I have to set the voltages for each comp control to minimize the histogram overlaps in the different channels. Then I alter the voltage in the correct channel to make that histogram have highest signal than all other channels and make a gate on that histogram.
My question:
After I am done with the compensation controls, when I run my actual samples, can I alter the voltage settings for each channel or do they have to be the same values as those set during the compensation.
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