Isolation of naive mouse T cells, should I positively select on CD62L after I negatively select?

Does anti-CD62L antibody lead to some form of signaling on the naive T cells?

07 August 2018 2,772 2 View

Why can AF488 and BV510 be used together?

I see that their emission spectrum overlaps entirely, but I've seen this combination being used, apparently successfully.

07 August 2018 4,900 6 View

Can I use anti-CD11c-PE and anti-TCRb-PE in the same staining panel since they are expressed on different cells?

Can I use anti-CD11c-PE and anti-TCRb-PE in the same staining panel since they are expressed on different cells?

06 July 2018 2,055 1 View

Protein Expression Level Reference Online for flow cytometry panel design?

Hello, I am looking for any online reference on any/all available protein expression data on any cell type to aid in the optimal design of flow cytometry panels. If anyone knows of any such...

01 February 2018 6,039 3 View

Setting voltage for compensation control vs actual samples?

I am newbie to flow cytometry. I use a LSR fortessa with FACSDiva software. When I run compensation controls, I have to set the voltages for each comp control to minimize the histogram overlaps...

11 December 2017 2,749 0 View

Not enough cells for FMO control, what's the alternative?

Is there an alternative to FMO controls if you don't have enough cells for FMO controls?

10 November 2017 8,443 3 View

I see "free acid" on HEPES bottle on some brands but not others, what does it mean?

see question

09 October 2017 509 0 View

Do FMO controls need same number of cells as actual samples?

I am flowing a lot of cells per sample

09 October 2017 2,419 3 View

What is the best digestive enzyme solution to digest mouse thymus for single cell suspension?

I want to look at dendritic cells by flow cytometry. I've been using Collagenase IV plus DNase I in blank RPMI1640 media.

06 July 2017 7,123 2 View

Does anyone have experience doing IHC for FFPE mouse ear? How do you keep the tissue from falling apart?

The blocks were cut into 6um sections and let float in 43C water bath before scooping up with microscope slide (time in water approx

31 December 2015 2,683 4 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 5,997 2 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View

Is there any expert in Smart PLS who can help me?

Dear colleagues, I have a manuscript that needs an expert in smart PLS to run the analysis, Is there anyone expert and ready to help me, please?

01 March 2021 8,212 3 View

Why initial concentration of Pb not the same as calculated one when testing using AAS?

I am currently doing research on the adsorption of heavy metal using magnetic reduce graphene oxide. I use AAS to determine the concentration of the heavy metal. I prepare the Pb solution with...

26 February 2021 9,580 4 View

How to run Cross-lagged panel Design on AMOS?

25 February 2021 3,126 1 View