Yong Lin

16 Questions 8 Answers 0 Followers

Questions related from Yong Lin

Isolation of naive mouse T cells, should I positively select on CD62L after I negatively select?

Does anti-CD62L antibody lead to some form of signaling on the naive T cells?

08 August 2018 2,847 2 View

Why can AF488 and BV510 be used together?

I see that their emission spectrum overlaps entirely, but I've seen this combination being used, apparently successfully.

08 August 2018 5,048 6 View

Can I use anti-CD11c-PE and anti-TCRb-PE in the same staining panel since they are expressed on different cells?

07 July 2018 2,120 1 View

Protein Expression Level Reference Online for flow cytometry panel design?

Hello, I am looking for any online reference on any/all available protein expression data on any cell type to aid in the optimal design of flow cytometry panels. If anyone knows of any such...

02 February 2018 6,108 3 View

Setting voltage for compensation control vs actual samples?

I am newbie to flow cytometry. I use a LSR fortessa with FACSDiva software. When I run compensation controls, I have to set the voltages for each comp control to minimize the histogram overlaps...

12 December 2017 2,840 0 View

Do I need to run compensation controls for the same panel every time?

I am quite new to flow so I was just wandering if I have to do it every time. Seems I can use previous compensation setting.

11 November 2017 3,613 2 View

Not enough cells for FMO control, what's the alternative?

Is there an alternative to FMO controls if you don't have enough cells for FMO controls?

11 November 2017 8,594 3 View

I see "free acid" on HEPES bottle on some brands but not others, what does it mean?

see question

10 October 2017 579 0 View

Do FMO controls need same number of cells as actual samples?

I am flowing a lot of cells per sample

10 October 2017 2,497 3 View

What is the best digestive enzyme solution to digest mouse thymus for single cell suspension?

I want to look at dendritic cells by flow cytometry. I've been using Collagenase IV plus DNase I in blank RPMI1640 media.

07 July 2017 7,211 2 View

Does anyone have experience doing IHC for FFPE mouse ear? How do you keep the tissue from falling apart?

The blocks were cut into 6um sections and let float in 43C water bath before scooping up with microscope slide (time in water approx

01 January 2016 2,800 4 View

My CHO-K1 grows in regular DMEM which does not have proline. Why?

see title

10 October 2014 8,154 0 View

In the case of hypoxia, what gas(es) becomes the dominant species that replace oxygen in the body?

I am guessing Carbon dioxide.

01 January 2013 1,919 17 View

Survey: Cell Culture Flask Disinfectant - Do/Should you use one?

Do/Should you spray/wipe the outside of an ongoing cell culture flask (i.e. with cell+culture media in flask) with any type of disinfectant before place into Biosafety Cabinet (BSC)? What do you...

10 October 2012 6,223 7 View

Do you routinely use antibiotics in culture media? What do you use?

Do you routinely use antibiotics in culture media? What do you use? What is your opinion on the fact that some cell culture experts recommend against routine use of antibiotics in culture media?...

10 October 2012 9,737 18 View

Isolating and maintaining primary rat bone marrow stromal cells

I have isolated primary rat bone marrow by cutting both ends of femur and tibia and flushing the bone marrow with media. After flushing, I filter the cell suspension through a 70um filter to get a...

09 September 2012 3,809 4 View