What is the best digestive enzyme solution to digest mouse thymus for single cell suspension?

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Isolation of naive mouse T cells, should I positively select on CD62L after I negatively select?

Does anti-CD62L antibody lead to some form of signaling on the naive T cells?

07 August 2018 2,879 2 View

Why can AF488 and BV510 be used together?

I see that their emission spectrum overlaps entirely, but I've seen this combination being used, apparently successfully.

07 August 2018 5,089 6 View

Can I use anti-CD11c-PE and anti-TCRb-PE in the same staining panel since they are expressed on different cells?

06 July 2018 2,150 1 View

Protein Expression Level Reference Online for flow cytometry panel design?

Hello, I am looking for any online reference on any/all available protein expression data on any cell type to aid in the optimal design of flow cytometry panels. If anyone knows of any such...

01 February 2018 6,142 3 View

Setting voltage for compensation control vs actual samples?

I am newbie to flow cytometry. I use a LSR fortessa with FACSDiva software. When I run compensation controls, I have to set the voltages for each comp control to minimize the histogram overlaps...

11 December 2017 2,910 0 View

Do I need to run compensation controls for the same panel every time?

I am quite new to flow so I was just wandering if I have to do it every time. Seems I can use previous compensation setting.

10 November 2017 3,650 2 View

Not enough cells for FMO control, what's the alternative?

Is there an alternative to FMO controls if you don't have enough cells for FMO controls?

10 November 2017 8,659 3 View

I see "free acid" on HEPES bottle on some brands but not others, what does it mean?

see question

09 October 2017 606 0 View

Do FMO controls need same number of cells as actual samples?

I am flowing a lot of cells per sample

09 October 2017 2,518 3 View

Does anyone have experience doing IHC for FFPE mouse ear? How do you keep the tissue from falling apart?

The blocks were cut into 6um sections and let float in 43C water bath before scooping up with microscope slide (time in water approx

31 December 2015 2,847 4 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is this a facetotecta nauplius?

This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.

08 August 2024 3,746 4 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View