Isolation of naive mouse T cells, should I positively select on CD62L after I negatively select?

Does anti-CD62L antibody lead to some form of signaling on the naive T cells?

07 August 2018 2,772 2 View

Why can AF488 and BV510 be used together?

I see that their emission spectrum overlaps entirely, but I've seen this combination being used, apparently successfully.

07 August 2018 4,900 6 View

Can I use anti-CD11c-PE and anti-TCRb-PE in the same staining panel since they are expressed on different cells?

Can I use anti-CD11c-PE and anti-TCRb-PE in the same staining panel since they are expressed on different cells?

06 July 2018 2,055 1 View

Protein Expression Level Reference Online for flow cytometry panel design?

Hello, I am looking for any online reference on any/all available protein expression data on any cell type to aid in the optimal design of flow cytometry panels. If anyone knows of any such...

01 February 2018 6,039 3 View

Setting voltage for compensation control vs actual samples?

I am newbie to flow cytometry. I use a LSR fortessa with FACSDiva software. When I run compensation controls, I have to set the voltages for each comp control to minimize the histogram overlaps...

11 December 2017 2,749 0 View

Do I need to run compensation controls for the same panel every time?

I am quite new to flow so I was just wandering if I have to do it every time. Seems I can use previous compensation setting.

10 November 2017 3,514 2 View

Not enough cells for FMO control, what's the alternative?

Is there an alternative to FMO controls if you don't have enough cells for FMO controls?

10 November 2017 8,443 3 View

I see "free acid" on HEPES bottle on some brands but not others, what does it mean?

see question

09 October 2017 509 0 View

Do FMO controls need same number of cells as actual samples?

I am flowing a lot of cells per sample

09 October 2017 2,419 3 View

Does anyone have experience doing IHC for FFPE mouse ear? How do you keep the tissue from falling apart?

The blocks were cut into 6um sections and let float in 43C water bath before scooping up with microscope slide (time in water approx

31 December 2015 2,683 4 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

No title

02 March 2021 3,060 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View