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Does anti-CD62L antibody lead to some form of signaling on the naive T cells?
07 August 2018 2,879 2 View
I see that their emission spectrum overlaps entirely, but I've seen this combination being used, apparently successfully.
07 August 2018 5,089 6 View
Can I use anti-CD11c-PE and anti-TCRb-PE in the same staining panel since they are expressed on different cells?
06 July 2018 2,150 1 View
Hello, I am looking for any online reference on any/all available protein expression data on any cell type to aid in the optimal design of flow cytometry panels. If anyone knows of any such...
01 February 2018 6,142 3 View
I am newbie to flow cytometry. I use a LSR fortessa with FACSDiva software. When I run compensation controls, I have to set the voltages for each comp control to minimize the histogram overlaps...
11 December 2017 2,910 0 View
I am quite new to flow so I was just wandering if I have to do it every time. Seems I can use previous compensation setting.
10 November 2017 3,650 2 View
Is there an alternative to FMO controls if you don't have enough cells for FMO controls?
10 November 2017 8,659 3 View
I am flowing a lot of cells per sample
09 October 2017 2,518 3 View
I want to look at dendritic cells by flow cytometry. I've been using Collagenase IV plus DNase I in blank RPMI1640 media.
06 July 2017 7,246 2 View
The blocks were cut into 6um sections and let float in 43C water bath before scooping up with microscope slide (time in water approx
31 December 2015 2,847 4 View
"PUBLISHING IN A SCOPUS JOURNAL" Researchers are now at a cross road. The critical need to publish in a Scopus or ISI, etc journal is ever vital. Journal Publication fees must be submitted....
10 August 2024 8,621 1 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.
07 August 2024 8,782 3 View
Please can anyone support with the survey questions based on RQ measures and propose how to do it in FMCG industry and include as well the role of brand equity Thanks
06 August 2024 949 0 View
Dear readers, Thanks for your attention. I am wondering about the health economic problem of quantifying the value of interventions which a) prevent, b) improve symptom profile and c) ultimately...
05 August 2024 3,246 1 View
I have an antibody binding generic protein and I need to compare its activity in a free and immobolized form. I understand that there are a number of methods to determine Kd value of a free...
05 August 2024 5,311 0 View
for eg. transition shock scale
01 August 2024 5,998 0 View
Because I have realized that the world tends more and more to do open and free science and there is a trend more and more to choose free databases, free tools and open access platforms.
01 August 2024 10,046 1 View
I am attempting to conjugate PEG to an amino acid at the C-terminus, for the purposes of producing nanoparticles. I have been told that PEG modified with amine groups can be used for this purpose,...
31 July 2024 2,033 1 View
Hi, researchers, Is it possible to use a safer acid instead of monochloroacetic acid to convert bacterial cellulose to carboxymethyl cellulose?
29 July 2024 4,570 0 View