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Does anti-CD62L antibody lead to some form of signaling on the naive T cells?
07 August 2018 2,772 2 View
I see that their emission spectrum overlaps entirely, but I've seen this combination being used, apparently successfully.
07 August 2018 4,900 6 View
Can I use anti-CD11c-PE and anti-TCRb-PE in the same staining panel since they are expressed on different cells?
06 July 2018 2,055 1 View
Hello, I am looking for any online reference on any/all available protein expression data on any cell type to aid in the optimal design of flow cytometry panels. If anyone knows of any such...
01 February 2018 6,039 3 View
I am newbie to flow cytometry. I use a LSR fortessa with FACSDiva software. When I run compensation controls, I have to set the voltages for each comp control to minimize the histogram overlaps...
11 December 2017 2,749 0 View
I am quite new to flow so I was just wandering if I have to do it every time. Seems I can use previous compensation setting.
10 November 2017 3,514 2 View
Is there an alternative to FMO controls if you don't have enough cells for FMO controls?
10 November 2017 8,443 3 View
I am flowing a lot of cells per sample
09 October 2017 2,419 3 View
I want to look at dendritic cells by flow cytometry. I've been using Collagenase IV plus DNase I in blank RPMI1640 media.
06 July 2017 7,123 2 View
The blocks were cut into 6um sections and let float in 43C water bath before scooping up with microscope slide (time in water approx
31 December 2015 2,683 4 View
I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...
03 March 2021 3,661 2 View
I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...
02 March 2021 4,443 2 View
So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...
02 March 2021 1,146 2 View
Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...
01 March 2021 8,766 3 View
during ultrasound examination of acute abdomen, sometimes we see free fluid , can we know the type of fluid ,whether blood or not , using ultrasound only? if yes , what are the sonographic...
01 March 2021 7,476 4 View
I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...
01 March 2021 9,015 2 View
I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??
28 February 2021 2,153 1 View
hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...
27 February 2021 3,882 3 View
I have run a MD simulation of a ligand-protein complex in GROMACS using CHARMM forcefield. Reviewer has asked me to calculate Ligand-activesite distance and binding free energy of ligand with...
27 February 2021 4,703 1 View
I am looking for a free software for text mining and sentiment analysis for my research on customer review mining (it involves calculating polarity of attributes,opinion oriented information...
25 February 2021 1,920 6 View
