MINOCYCLINE safe to use 3 months after expiry?

I plan to call my PCP tomorrow to refill it but I had some left over from a spinal infection (which reoccurs) so I took the initial dose today. Its 3 months past its expiry date. I've seen that it...

30 July 2020 9,983 7 View

Why arn't small MW proteins transferring?

25 August 2016 7,374 11 View

Does anyone regularly use a vaporizer to administer isoflurane for anesthetizing mice?

I'm trying to anticipate how much isoflurane I will be using in a year. I will be anesthetizing about 200 mice for about 10 minutes each mouse over the course of a year. Can anyone approximate how...

09 December 2015 2,501 2 View

Why am I getting a peak at the same temperature as my samples for my NEC?

I continually get peaks in my no enzyme control for real time PCR in my melt curve. Though, my Cts are ~7 cycles away. I have DNase treated my RNA twice, changed all reagents and decontaminated...

28 October 2015 6,334 2 View

Would you like to collaborate on a medical device regulatory book contracted through a reputable organization?

03 April 2015 8,554 8 View

Can anyone recommend a general anti fungal drug to add to a bacteria media to avoid contamination?

13 October 2014 152 10 View

Why is the supernatant from my pET expression system lysis step so viscous?

I have been expressing and lysing this construct for years, and today I got a thick gel instead of a nice supernatent. I recently started using Magic Media instead of IPTG induction, but this has...

05 September 2014 5,403 4 View

Which breast cancer cell lines do you find grow best in nude mice?

I have worked exclusively with triple negative MDA-MB-231. We are thinking about redoing projects using a different TNBC cell line or ER+ line such as BT20 or MCF7, respectively. The xenograft...

07 July 2014 8,830 6 View

Domo for decision-making?

I have seen several ads referring to DOMO as a better method for tracking data vs. Excel Spreadsheets because it has the capacity to inform decision-making. Has anyone seen a demo and/or used the...

06 July 2014 7,556 2 View

How long can fresh frozen tissue be stored at -80C?

I need to store fresh frozen tumor tissue from nude mice for western blotting, receptor binding assays, and immunohistochemistry. In our lab, we freeze tissue for western blots in RIPA buffer, in...

14 May 2014 1,481 9 View

How to compare different concentrations of nanoparticles for characterization from ICP-MS?

I took measurements of different concentrations of 15 nm and 5 nm Au nano particle solutions. I'm trying to determine which concentration I measured gives the best reading overall. I also...

02 March 2021 7,626 1 View

What are the new 2D nanomaterials were used for Silver nanoparticles stabilization?

In the stabilization of AgNPs are vital in current research interest. However, some of the 2D nano-materials like graphene oxide, MoS2, etc... previously used to stabilize it. But still they shown...

01 March 2021 2,207 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Why there are two peaks while using the mie plot of Silver nanoparticle?

Hi, I am trying to learn the theoretical modelling of silver nanoparticle using the Mie scattering theory. After that, I discovered a software named Mieplot developed by Philip Laven, where it...

25 February 2021 7,493 6 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View