Richelle Galope

9 Questions 8 Answers 0 Followers

Questions related from Richelle Galope

Unusual case during stability study in ADC formulation? Contamination or protein degradation?

Hi all, For context, I am conducting a stability study using an ADC material formulated in different buffer systems to screen for the optimal pH/buffer to use for the final drug formulation. The...

24 November 2023 5,451 2 View

Aside from the molecular pI, pH conditions and resin type, what other factors affect the binding of a molecule to ion exchangers?

I am fairly new to ion exchange chromatography, and I am trying to optimize conditions to purify a protein having a pI value 8. I am using an anion exchanger (Q) in pH 6.0. I am aiming to separate...

11 November 2019 5,243 3 View

Ho can I achieve a consistent purification result using Ni-IDA resin?

I am purifying a protein naturally containing His residues (the number of residues are much lesser than the His-tagged ones so binding is a bit weaker). I have previously established a...

28 March 2019 9,443 5 View

MBP+signal peptide (periplasmic space) vs. SOX+disulfide isomerase?

Hi! Can anyone give insights as to which of these two methods is better? We're trying to express a recombinant protein in E.coli. It is quite difficult to express this protein in its native...

06 August 2018 8,930 1 View

Possible reasons for the difference in size of the standard and recombinant protein?

We expressed a recombinant protein in yeast. This protein naturally has one glycosylation site, but site-directed mutation was done to produce a non-glycosylated form since yeast is able to...

08 February 2018 4,134 2 View

Refolding condition for TGF beta-3?

Hi, can anyone share a good refolding method for TGF beta3 expressed in E.coli as inclusion bodies? We have tried using a refolding screen test kit from Pierce, but we get protein...

16 August 2017 4,497 3 View

Is there a standard acceptable value of sequence coverage for peptide mass fingerprinting?

After doing 2DGE and MALDI-MS, some of the proteins identified have lower sequence coverage values (

21 December 2016 922 7 View

Can anyone suggest a good protein precipitation protocol which is compatible with co-IP?

I am working on a secreted protein, so my protein samples are taken from the cultured media. I wanted to check interaction of this protein with other proteins also, so I am planning to do...

25 August 2016 1,048 3 View

How do we know how many fold a particular protein is induced or repressed? this?

This question is concerned with the study about interferon response in human hepatoma cell line (Huh7). After doing proteomic analysis using ICAT and tandem mass spectrometry (MS/MS), this data is...

22 April 2015 9,042 2 View