Hello everyone,
I'm planning a study that involves treating mice with the PPAR gamma agonist rosiglitazone. It seems like I have a few options for administering Rosi, including gavage, ip injection, or as a dietary supplement. I couldn't seem to find any literature describing how to impregnate the chow with rosiglitazone. I apologize if this is an ignorant question, but I've never done it before and don't know whether I need a solvent or if I just.. sprinkle it on there. Furthermore, I'm not quite sure of the advantage to adding bioactives to chow compared to ip injection. Obviously with ip you ensure dosage, but in your experience does diet supplementation still work effectively without the need to handle all the animals daily? Thanks for your time!
I doubt that your animal ethics committee will approve gavage or injection if adding the substance to the diet is possible. Is the substance heat labile? Then it should be sprayed on the pellets. If not, then it can be adder dry or in a solution before pelleting.
Hi Beth Worley !
I did an experiment years ago administering rosiglitazone in rodent chow. By that time, I had experience with drugs delivered in the water and orogastric gavage, and I wanted to try another route because of some limitations I had encountered in the former routes.
First, I searched for the dose (mg/kg BW). Then, I estimated the average food intake (g/day) to know how much drug I should add per g of diet. Finally, I bought the drug and send to the company that prepares my purified diets.
It worked nice for rosiglitazone and the other 2 drugs that I used, but it has some limitations.
The first issue is that food intake varies, and on each day, mice will ingest a different amount of food and thus a different medication dose. In addition, if the animal gain or loose weight and does not change food intake, drug doses also change.
Here are the papers with rosiglitazone:
Article Pan-PPAR agonist beneficial effects in overweight mice fed a...
Article Rosiglitazone Aggravates Nonalcoholic Fatty Pancreatic Disea...
In this paper we discuss routes for drug administration:
Article Telmisartan detection by UV spectrophotometry in mice drinking water
Hope this helps!
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