Beth Caruana

4 Questions 6 Answers 0 Followers

Questions related from Beth Caruana

Why arn't small MW proteins transferring?

Hi, Three times when I have transferred proteins from a 10% SDS-PAGE gel to a nitrocellulose membrane proteins smaller than 50kDa weren't detected. I have used fresh gels, changed the current,...

26 August 2016 7,554 11 View

Why am I getting a peak at the same temperature as my samples for my NEC?

I continually get peaks in my no enzyme control for real time PCR in my melt curve. Though, my Cts are ~7 cycles away. I have DNase treated my RNA twice, changed all reagents and decontaminated...

29 October 2015 6,451 2 View

Why arn't my bands staining brown when I silver stain?

I am silver staining a gel and the bands appear clear instead of brown. Does anyone know what could be causing this?

15 May 2015 9,622 4 View

Why is my gel not staining in the middle?

I have loaded a 16.5 % tricine gel with protein, and for my last 4 gels, it appears that a section of the gel is unstained in the bottom middle of the gel after I silver stain. Does anyone know...

29 April 2015 4,646 5 View