Does any know the difference between psPAX and PAX2 LV plasmid?

I want to know which one is more suitable for dendritic cell transduction. I see that in: https://www.cell.com/cell/pdfExtended/S0092-8674(15)00829-6 PAX2 plasmid is used for dendritic cells...

31 December 2018 3,696 3 View

Is there a difference between human and mice codon optimised sequences?

I have a Cas9 plasmid which is human codon optimised. Will there be any difference in Cas9 protein expression if this is expressed in mice?

07 August 2018 5,808 0 View

How to add +/- signs in front of calculated mean value in Excel?

I'm not referring to error bars. I want to add +/- signs in front of my mean (just like in a paper), without having to go to insert symbols and inserting them individually. Are there any keys to...

10 November 2013 8,421 9 View

Is SOD and CAT activity present after 24h of autopsy provided its stored at -20C?

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09 October 2013 5,827 1 View

In which solvents are beta-carboline alkaloids soluble?

I tried it with water, 5% DMSO but it seems to insoluble in those solvents.

09 October 2013 6,216 7 View

Identifing the epididymal luminal protein/s in lizards?

08 September 2013 2,651 4 View

TLC trouble shooting

I'm running TLC with silica sheets. As the solvent travels quarter way up its movement changes such that we no longer see a straight line of solvent but rather a squiggly line. This largely...

08 September 2013 7,048 15 View

What are your thoughts on my observations of STZ in fed and fasted conditions?

What should be the nutritional status of wistar rats before the injection of STZ.? I came across this very interesting paper titled 'Streptozotocin is equally diabetogenic whether administered to...

07 August 2013 6,918 1 View

what does 30% w/v of H2O2 mean?

what does 30% w/v of H2O2 mean? does it mean 30g of H2O2 is present in 100ml of that solution?

07 August 2013 529 17 View

Calculation help for TBARS

I'm getting 1731nM of MDA in my 10% homogenate. And my 10% homogenate has 16.44 mg/ml of protein. How do I express my results in terms of nmoles of MDA/mg of protein? It should fall within the...

07 August 2013 471 16 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

I have designed reflectarray element and feeding it though Horn Antenna on HFSS. After full wave simulation, I am not getting desired results?

I have designed reflectarray element to generate OAM beams at 5.8GHz and feeding it though Horn Antenna on HFSS. After full wave simulation, i am not getting desired results i.e Radiation pattern...

01 March 2021 9,701 5 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View