Does any know the difference between psPAX and PAX2 LV plasmid?

I want to know which one is more suitable for dendritic cell transduction. I see that in: https://www.cell.com/cell/pdfExtended/S0092-8674(15)00829-6 PAX2 plasmid is used for dendritic cells...

31 December 2018 3,696 3 View

How to add +/- signs in front of calculated mean value in Excel?

I'm not referring to error bars. I want to add +/- signs in front of my mean (just like in a paper), without having to go to insert symbols and inserting them individually. Are there any keys to...

10 November 2013 8,421 9 View

Is SOD and CAT activity present after 24h of autopsy provided its stored at -20C?

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09 October 2013 5,827 1 View

In which solvents are beta-carboline alkaloids soluble?

I tried it with water, 5% DMSO but it seems to insoluble in those solvents.

09 October 2013 6,216 7 View

Why are standards for phenolics dissolved in methanol for HPLC?

Why are standards for phenolics dissolved in methanol for HPLC?

09 October 2013 350 9 View

Identifing the epididymal luminal protein/s in lizards?

08 September 2013 2,651 4 View

TLC trouble shooting

I'm running TLC with silica sheets. As the solvent travels quarter way up its movement changes such that we no longer see a straight line of solvent but rather a squiggly line. This largely...

08 September 2013 7,048 15 View

What are your thoughts on my observations of STZ in fed and fasted conditions?

What should be the nutritional status of wistar rats before the injection of STZ.? I came across this very interesting paper titled 'Streptozotocin is equally diabetogenic whether administered to...

07 August 2013 6,918 1 View

what does 30% w/v of H2O2 mean?

what does 30% w/v of H2O2 mean? does it mean 30g of H2O2 is present in 100ml of that solution?

07 August 2013 529 17 View

Calculation help for TBARS

I'm getting 1731nM of MDA in my 10% homogenate. And my 10% homogenate has 16.44 mg/ml of protein. How do I express my results in terms of nmoles of MDA/mg of protein? It should fall within the...

07 August 2013 471 16 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View