I'm getting 1731nM of MDA in my 10% homogenate. And my 10% homogenate has 16.44 mg/ml of protein. How do I express my results in terms of nmoles of MDA/mg of protein?
It should fall within the range of 4-20nmoles of MDA/mg of protein.
It means
16.44 mg/ml of proteins have 1731 nM of MDA
And 1 mg/ml contains...............................1731/16.44
i.e,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,105.29 Nm
so this value will be 105.29 nM/mg ml
Why are you expressing 1731nmoles in pico moles........... Its actually in nano moles?
Ok......I'm getting two contradictory answers....... 0.11 nmol/mg protein and 105.29nM/mg protein. which one is it?
Ok... I'll give you the correct data
1731nmoles of MDA in 166micro liters of 10% homogenate &
16.44mg/ml in 25 microliters of 10%homogenate
how do I express this in nM MDA/mg protein
nM MDA/mg protein makes no sense, you're mixing concentration with amounts of protein. Your numbers are a bit strange too, as your concentration of MDA in your homogenate is about 10 mM (massive) and recalculated into amount/mg protein it is about 600 nmol/mg protein. Something is wrong. How did you measure the nmoles of MDA in 166 ul?
dosen't it come to 15.8nMoles/mg of protein?
if 25 microliters has 16.44mg of protein then 166micro liters has 109.16mg of protein.
hence 1731/109.16 will give 15.8nMoles/mg protein.
You said there was 16.44 mg/ml protein concentration, that's a different thing from 16.44 mg of protein in 25 ul. If the latter were true, protein concentration would be 657 mg/ml (16.44/0.025). Your calculation would work if you really have 1731 nmol/166 ul homogenate. How did you measure it? Absorbance/fluorescence?
my samples are one month old.......does that make any difference. I had kept them in deep freeze
with the addition TCA-TBA-Hcl solution. Boiled it for 15 mins. The pink colour was read at 537nm. 1,1,3,3 tetra methoxy propane was taken as the standard
Right, so from the calibration curve you get the *concentration* of MDA in your sample, e.g. 1731 nM. You take that a calculate the *amount* of MDA in 1 ml, which is 1731 pmol or 1.731 nmol. Since there is 16.44 mg of protein in one ml, you divide the two numbers and get the amount of MDA per mg protein.
- Badges
- Science topic
- Similar topics
- Medicine
- Disease
- Metabolic Diseases
- Glucose Metabolism Disorders
- Diabetes