what does 30% w/v of H2O2 mean? does it mean 30g of H2O2 is present in 100ml of that solution?
Dear Indumati Sharma,
That is exactly what it means, 30% w/v of hydrogen peroxide means for each 100 mL of solution there are 30 g of hydrogen peroxide.
Dear Nor Hazwani Md. Zain,
The difference between "weight" percentage and volume percentage, besides what it literally means (the weight is in fact mass (grams) not weight (newtons), volume is just volume) the difference is the material's density, which for hydrogen peroxide is about 1.45 g/cm3 (at 20ºC). The density is the mass of a specific material for each cubic centimetre of space (volume).
Best regards,
Sebastião
Dear Indumati,
% w/v means "weight per volume", i.e. 30g H2O2 per 100mL (of whatever solvent).
It's a bit unusual though as normally you use this as 30%w/w (or wt %, i.e. weight %) - that's also the way you can buy it (http://www.sigmaaldrich.com/catalog/product/sial/216763?lang=en®ion=GB).
Good luck.
30% (w/v) means: if you take 100 ml of that solution, it contains 30 g of H2O2.
You should keep it at 4°C in a dark bottle.
H2O2 is an oxidizer. It gets easily decomposed when exposed to light. So, I would suggest you to purchase H2O2 solution, which is readily available in market as 30% solution only. Or, you keep the solution you are making in dark in black bottles/containers to use it for long (more than a month). It will remain..
My 30% w/v h2o2 didn't last even for a month. I had opened the bottle , dark bottle, for at least four times in a month and now I'm not getting any reading for catalase assay. However I didn't store it in 4'C. Could this be the reason_?
What is the difference between weight percentage and volume percentage if i have a solution containing 30% hydrogen peroxide? Is the 30% hydrogen peroxide available in the market is in powder form or solution form? I am confused.
Dear Indumati Sharma,
That is exactly what it means, 30% w/v of hydrogen peroxide means for each 100 mL of solution there are 30 g of hydrogen peroxide.
Dear Nor Hazwani Md. Zain,
The difference between "weight" percentage and volume percentage, besides what it literally means (the weight is in fact mass (grams) not weight (newtons), volume is just volume) the difference is the material's density, which for hydrogen peroxide is about 1.45 g/cm3 (at 20ºC). The density is the mass of a specific material for each cubic centimetre of space (volume).
Best regards,
Sebastião
Generally, SI unit is preferred, but one may follow any unit to pursue the research; that is not going to decide the failure or success of the experiments...
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