Do salt in PBS affect the TEM image quality ?

If I prepare my sample in PBS, do the salt in PBS will affect the TEM image quality?

04 October 2019 9,331 3 View

Reduced mitochondrial as a protection for neurodegenerative diseases?

Mimitochondrial fission is generally regarded as getting rid of bad mitochondria, perhaps the increased fission is trying to get rid of poor mitochondria and what is the purpose of reducing...

27 August 2016 7,934 4 View

What are the applications of Taguchi Loss function in the field of automotive engineering? Do OEMs use loss function in design or manufacturing stage?

I am interested in knowing if there are any journals or papers which discuss about the applications of Taguchi Loss function in Automotive Industry.

15 April 2016 1,389 1 View

Can you any one suggest good antibody for detecting pTau by IHC ?

I wanted to detect pTau 181or ser202/Thr205 in APP and Tau tg mice. Here I want to suggestion for good primary antibodies (companies and cat #) to detect ptau in these mice sections by IHC.

29 March 2016 8,377 4 View

How to measure Early Voltage of a BJT experimentally?

Hi All, Could you please explain me how to measure the Early Voltage of a BJT experimentally in lab (Undergraduate Electronic Devices Lab)? I am also interested to know the different parameters of...

22 December 2015 5,701 5 View

Doesn't water affect the RAW 264.7 cells?

To induce inflammation in RAW 264.7 cell lines, we treat cells with LPS (lipopolysaccharide) which is supposed to be dissolve in water. Did adding of water to the culture have any effect on cell...

03 February 2015 5,895 4 View

Can anyone quantify IL-6 or TNFa expression from immunofluorescence microscopic images of cultured cells?

Is image J software the right one or is some other software helpful?

12 April 2014 3,225 6 View

Does anybody suggest to reduce cytosolic staining along with nucleus in the cells stained with vectashield-DAPI reagent for fluorescent micrscopy?

26 December 2013 7,532 1 View

Why is it assumed Z=e^jw and S=jw?

20 December 2013 5,508 21 View

Signal in water samples when I do Real time PCR.

When I do real time PCR, sometimes I get an amplification signal in the water sample. I use AB systems Fast SYBR Green master mix (Cat NO: 4385612). I setup the experiment along with house...

12 February 2013 9,804 8 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View