If I prepare my sample in PBS, do the salt in PBS will affect the TEM image quality?
Salts form crystals, which may overlay your specimen.
Good morning,
after fixation you have to wash with water before dehydration. without washes, it may happen to observe samples with saline precipitates
best regards
Hello,
following, the link to a previous discussion about PBS/cacodylate buffer in EM:
https://www.researchgate.net/post/Is_cacodylate_buffer_necessary_in_SEM_protocol_or_I_can_remove_it
I agree with Simone Cantamessa, If you use PBS, you need good washes with water to avoid crystals.
Hi, I am interested to know if there is any relationship between Static Pull-out (Release) voltage and Dynamic Pull-out (Release) voltage in fixed-free MEMS cantilever. In literature, I find that...
20 April 2019 5,114 0 View
Hi all, Consider two capacitors, say "C1" and "C2" connected in series. And a voltage source "V" is applied. How can the charge "Q" be calculated practically? I understand that the charge "Q"...
31 March 2019 1,290 11 View
Could you please let me know the reason for the oscillatory response of the MEMS electrostatic cantilever beam when released (i.e., when voltage is removed)? I understand that a similar "bouncing"...
03 January 2019 3,827 5 View
Dear all, Please help me to obtain the charge-voltage chara of a capacitor in circuit simulator (Like LTSpice / QUCS). The simulator gives the voltage across and current through the capacitor....
27 May 2018 9,205 3 View
Dear all, Please provide some good help / reference documents or texts to understand the Finite Element Analysis using getDP tool .It would be great if anyone can provide the detailed...
13 March 2018 7,201 3 View
Dear all, 1) I am a new to the area of Finite Element Analysis. I want to understand the Finite Element formulation for "MEMS Electrostatic Actuator" that can be applied to any FEA tool (say any...
06 March 2018 9,525 3 View
Hi all, Please suggest me different CAD tools available for MEMS that will allow me to integrate other devices with MEMS (device description is based on differential equations) to study the...
26 February 2018 985 4 View
Hi all, Can you please explain the "Dynamic Pull-in" concept in electrostatic parallel plate MEMS actuators? I would like to know "in detail" about its causes and effects. How is it related to...
16 January 2018 1,996 2 View
Mimitochondrial fission is generally regarded as getting rid of bad mitochondria, perhaps the increased fission is trying to get rid of poor mitochondria and what is the purpose of reducing...
27 August 2016 8,017 4 View
I am interested in knowing if there are any journals or papers which discuss about the applications of Taguchi Loss function in Automotive Industry.
15 April 2016 1,537 1 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
Hey there, As a synthetic chemist delving into theoretical calculations for my imidazolium-based organic molecules, I would greatly appreciate any guidance on the appropriate input instructions...
09 August 2024 5,444 7 View
If we map as a continuous motion an ionising electron (beginning its journey at n=1) in an H atom, a specific hyperbolic spiral appears (see animation). When we solve this spiral formula, we find...
07 August 2024 5,343 2 View
Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...
07 August 2024 9,563 0 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
average particle size calculation from TEM
04 August 2024 2,921 1 View
Hi everyone I need a file with a dirty and clean potato image
04 August 2024 7,199 4 View
If from a geometric perspective the non-halogens, non-noble gases have more empty spots in their valence shell, and the filling/exiting of any of the empty spots in the shell constitutes a...
03 August 2024 4,787 2 View
Why is the molecule's orientation with an electric field affect polarizability? Electrons are diffuse enough to be independent with respect to orientation and effect of electric field on...
03 August 2024 7,843 1 View
I'm guessing it's because the ligand experiences too much electron repulsion or proton repulsion from the chromium to insert them close to the 3d-orbitals which are close to the metal nucleus. Is...
03 August 2024 1,370 1 View