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Questions related from Raghuram Kandimalla
If I prepare my sample in PBS, do the salt in PBS will affect the TEM image quality?
05 October 2019 9,464 3 View
To induce inflammation in RAW 264.7 cell lines, we treat cells with LPS (lipopolysaccharide) which is supposed to be dissolve in water. Did adding of water to the culture have any effect on cell...
04 February 2015 6,024 4 View
We cultured RAW 264.7 cells in DMEM medium, but there is no uniform growth in culture flask, all cells are grown as clumps at one place (and many like that through out the plate), there is no...
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I have to select a protease inhibitors cocktail in western blot analysis of nf-kb and cox-2. Can I use protease inhibitors from roche directly or i have to go for EDTA free protease inhibitors?...
23 January 2015 5,413 3 View
If a drug shows good anti inflammatory response in LPS induced inflammation in RAW 264.7 Cell lines and good hypoglycemic response in diabetic rats, does it work against diabetic neuropathy?...
20 January 2015 1,341 3 View
We bought DMEM with high glucose, but someone told us that it is toxic to cells! If we reduce the concentration of media does it work?
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