Why am I getting colonies in negative control plate after BCG electroporation?

07 December 2021 0 2K Report

Hi all,

I have been performing BCG electroporations and I am experiencing WT (control) colonies growing in 7h9 kan25ug/ml agar after electroporation. I have picked those colonies along with my recombinants and have transferred to 5ml of 7h9 kan 25 broth. The colonies grow well in broth also. How do i screen my recombinants and how to avoid this?

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