What commercially available enzyme can be used as a positive control for ATPase assay?

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Adam B Shapiro

Hexokinase: glucose + ATP glucose-6-phosphate + ADP

http://www.worthington-biochem.com/HK/cat.html

To detect the ADP, couple the reaction to pyruvate kinase (PK) + phosphoenolpyruvate and lactic dehydrogenase (LDH) + NADH. The ATP is regenerated by the PK reaction.

A convenient mixture of PK and LDH in 50% glycerol is commercially available.

Wasim Ahmed

Agreed with Adam Shapiro

Soundarya Priya

Thank you Adam B Shapiro