during my plant tissue culture experiment my agar is not solidifying, can anyone tell me why this happening, i followed all the step correctly.
agar not solidifying is common problem faced when ph set is not proper. ph changes by 0.4 after autoclaving. so u need to consider that margin as well
What's the medium and pH value? Complete MS medium or half strength MS. Give some more details.
agar not solidifying is common problem faced when ph set is not proper. ph changes by 0.4 after autoclaving. so u need to consider that margin as well
The pH value decrease by 0.3 after autoclaving. You need to increase the pH value to 5.83, this value is fit for most plant species.
For a complete MS Medium, pH could be from 5.5-5.8. Upon autoclaving, pH gets reduced (upto 4.4 if I am right). Please refer the attached paper
Hello, i also has the same problem but im using VW media. The pH value is between 5-6 i cant tell the exact pH because i only use pH paper mercks. Should i adds more NaOH when strring until its pH hits 6?
Use 0.7% PTC grade agar and adjust the pH of the media at 5.7 to 5.8 before add agar. I think media will be solidified. Remember that during autoclave the pH of the media reduced by 0.3, so, the pH of the media should not less 5.7.
Bosco Lawarence Maybe adding few drops HCl until the pH reach 4.5 will work
Bosco Lawarence Use agarose instead of agar...it is suitable for lower pH
I also had same problem, I used DKW medium with 2 g/l phytagel, pH medium before adding phytagel was 5.8, but could not be solified...
I use 8 gr/L for tissue culture medium and I have to check the pH first at 5.8 before autoclaving. Lower pH will make the medium not solidified.
Agar cannot solidified when the pH of the medium is lower than 5
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