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Dear fellow Researchers, I am currently trying to analyze Affymetrix microarray data through dChip software and I have the input files - probe sequence and CDF for Rat 230 2, yet facing issues in...
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Dear Researchers, I'm trying to construct a vector (using pcDNA3.1) with two different reporter genes with two different promoter sequences of target genes. I have two different constructs with...
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Dear Researchers, I have purchased my designed oligos from Sigma for miR based silencing studies as sense (~64nts) and antisense (~64nts); together if annealed it is ~64bp. I take equimolar...
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MCF-7 cells were already handled perfectly and were good. Currently, we are meeting fungal contamination within the next passage. There are no much possibilities of handling errors as we do have...
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3T3-L1 cells grow healthily when the humidity of the incubator is maintained by using a tray of water but it grows unhealthily and slow when the copper sulphate solution is used to maintain...
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3T3-L1 cells are insulin sensitive but when they are counted and seeded for insulin stimulated glucose uptake assay, it results with 123% of glucose being up-taken by the positive control cells...
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While running on 2% AGE (with product size ~320 bp), the negative control (Plasmid without insert) and the PCR control (without template) show bands with less intensity where there are no bands in...
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