Snehaa Chandrakumar

9 Questions 44 Answers 0 Followers

Questions related from Snehaa Chandrakumar

Where can I find the Gene info file to analyse my Affymetrix microarray data through dChip?

Dear fellow Researchers, I am currently trying to analyze Affymetrix microarray data through dChip software and I have the input files - probe sequence and CDF for Rat 230 2, yet facing issues in...

09 March 2020 7,785 3 View

Can a vector have two same poly A sequence in two different directions?If not, can the poly A sequence corresponding to antibiotic resistance be used?

Dear Researchers, I'm trying to construct a vector (using pcDNA3.1) with two different reporter genes with two different promoter sequences of target genes. I have two different constructs with...

31 January 2018 8,502 4 View

Difficult to figure out the concentration range for customizes oligos?

Dear Researchers, I have purchased my designed oligos from Sigma for miR based silencing studies as sense (~64nts) and antisense (~64nts); together if annealed it is ~64bp. I take equimolar...

09 January 2018 8,045 4 View

Reasons for yellow colored pellet during competent cell preparation??

Dear Researchers, I could see the pellet yellowish during the centrifugation steps in preparing E.Coli DH5 alpha cells for transformation. I would like to know the reasons for the color change...

15 June 2017 5,901 0 View

Can anyone help me in ruling out the issues in cloning siRNA into mammalian vector?

Dear Researchers, I have been trying to clone my siRNA which has miR155 backbone into modified pcDNA3.1. I start with annealing of oligos then restriction of vector (1ug/ ul vector conc; 20ul...

25 October 2016 9,344 2 View

What are the possibilities of fungal contamination apart from handling?

MCF-7 cells were already handled perfectly and were good. Currently, we are meeting fungal contamination within the next passage. There are no much possibilities of handling errors as we do have...

11 September 2015 4,279 11 View

Does copper sulphate influence the growth of 3T3-L1 cells incubated in CO2 incubator?

3T3-L1 cells grow healthily when the humidity of the incubator is maintained by using a tray of water but it grows unhealthily and slow when the copper sulphate solution is used to maintain...

07 February 2015 5,704 3 View

Is it acceptable to get a percentage value higher than 100 for my glucose uptake assay in 3T3-L1 cells?

3T3-L1 cells are insulin sensitive but when they are counted and seeded for insulin stimulated glucose uptake assay, it results with 123% of glucose being up-taken by the positive control cells...

07 January 2015 7,353 6 View

Could anyone suggest the reasons for visibility of showing low intense bands seen in PCR control (without template) compared to Test templates in PCR?

While running on 2% AGE (with product size ~320 bp), the negative control (Plasmid without insert) and the PCR control (without template) show bands with less intensity where there are no bands in...

01 January 1970 5,073 3 View