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Questions related from Sansrity Sinha
I used MEGA5.0 for computing MER for my dataset. the rates were computed based on JTT with an account of invariant sites model using 2 gamma categories for all positions in the alignment. these...
24 August 2018 4,415 1 View
I am looking for a tool that could predict the ancestral sequence for the phylogenetic tree. I have heard about FastML and MEGA6.0. Can anyone help me understand what do the alphabets M, L etc at...
17 December 2016 9,477 2 View
generally bacteria is used for protein expression in purification whenever protein is required in large quantities for structural studies. Is it possible to get such large amounts of proteins from...
13 August 2016 562 6 View
my dataset consists of highly similar sequences of proteins. i only wanted to remove duplicate sequeces if any in the data set. what threshold shall I set in CD HIT?
03 April 2016 4,614 1 View
i wanted to create a stable knock don cell line for my gene of interest. i chose 3'UTR targetting shRNA cloned in a retroviral vector for that purpose. I am using pSuper as entry vector and pUMVC...
01 March 2016 9,146 0 View
I wanted to make a stable cell line that expresses an shRNA. So I cloned shRNA in a retroviral vector. I wanted to know if I can know where exactly would it integrate in cell genome as it...
03 January 2016 3,809 1 View
i wanted to delete a strech of 120 amino acids present between N and C terminal of my protein. is i possible to remove such a long stretch by quick change method? any specific consideration for...
26 August 2015 3,243 4 View
I am working on a protein family that has two members. I tried to look up homologs and orthologs in lower organisms. i found two hits for search indicating presence of both genes in lower...
08 August 2015 1,687 9 View
hi, i have been trying to clone a fragment into pGEX6P1 vector. every time i amplify the insert (1.5kb), digested it with BamH1 and XhoI (sites flanking the insert), digest the vector too (since...
30 July 2015 4,475 3 View
i am working on two proteins each translated from two different genes belonging to same gene family. A closer look at the sequence tells me that the proteins differ in the number of charged...
30 July 2015 7,789 4 View
I am working on mitofusins which are of two types in humans (not isofoms, but two different genes encoding them). i want to know at what time in evolution did the duplication happen. also which...
18 June 2015 5,337 5 View
I have my gene of interest cloned in mammalian expression system. there are two gene that i am looking for. i have cloned each of them in two different plasmids both having different promotors of...
10 June 2015 9,667 3 View
can commonly used cell lines like HEK293 or HeLa not be used for this purose?
02 June 2015 3,361 1 View
While performing promotor analysis what roughly is the length of sequence considered to predict putative TF binding site? since I am new to this, I don't know. Also what should be the cut off...
13 May 2015 2,532 7 View
what surface markers are used to differetiate between a normal stem cell and CSCs and CTCs?
07 May 2015 5,455 5 View
although ADP is abundant in the cell and most reactions require ADP? does that has and additional evolutionary significance?
30 April 2015 10,022 3 View
