I am working on 3T3 cell lines. i grew it using DMEM in flask, then I transferred it to the 96 wells cell culture plate followed by cell counting and calculations. the cells remained fine till 2 days, meanwhile I changed the media as well during this incubation. but when I added the solution of MTT dye and DMEM after removing its media, the cells pellet become detached from the wells, showing detached aggregates of formazane. i could not know its reason!!!!! I request you to help me in this regard.
Thank You!