Hi all,

Kindly I have questions about peptide design. I'm now in the process of designing a peptide based on protein structure and the length of peptide is 45 a.a . I have predicted the structure by many tools but my problem the two catalytic residues in peptide are not located in front of each other so kindly is there any advice or tool we can detect the two catalytic residues in peptide before build the structure. Or is better to use scaffold? if yeah are there any mini or small scaffold for peptide.

The picture attached below is just to make it clearer (this is the protein that has catalytic residues, I need to make two catalytic residues of my peptide in front of each other)

thanks