Who can help me in the dentification of such strange structures in common sole stomach contents?

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Is there a way to draw and save multiple free-hand ROIS as TIFFs while also saving the .roi files?

Hi All, I’m new to ImageJ image processing and have a question about drawing multiple custom ROIs on an image and saving each ROI as a distinct TIFF. I’m working with images of a rodent brain,...

31 October 2023 1,355 0 View

Any particular advice to perform siRNA on cGAS with smartpool ON-TARGET?

I am trying to perform a siRNA on cGAS gene on HACAT cells and also HCT116 as control. I am using a smartpool purchased from Dharmacon (ON-TARGETplus siRNA MB21D1) following the manufacturer's...

12 April 2023 9,511 0 View

After extraction of cumin from tomato peels, is it possible to solubilize in any solvent?or can it only be dispersed in a liquid medium like H2O/EtOH?

How is it possible to obtain a more or less homogeneous solution containing cutin?

21 March 2023 5,212 0 View

Which is the best free software for animal photos tracking and behavioral studies?

Hello, I'm running a research on wild mammals with camera traps (capture mode: photo).I 'd like to analyse the data using tracking software to get information about animals activity (time spent...

27 July 2020 5,447 1 View

Is there any expert of mysids who can help in identifying an unknown species collected in the Adriatic?

I'm looking for an expert in Mysida whoc an help me in identifying a species (probably a Gastrosaccus) but that presents two spines on the carapace, I didn't find in the bibliography I have and...

03 March 2019 2,015 4 View

Where can I purchase LAD-2 cell line?

Is this cell line commercially available? Thank you for your help.

08 August 2018 5,786 0 View

PUFAs in cell colture medium and serum?

Does anyone know if in the cell colture medium and in the serum (FBS) are present PUFAs (polyunsaturated fatty acids)? I used both from SIGMA. Did someone perform mass spectrometry analysis on...

07 February 2017 194 3 View

Does anybody have antibodies for any fructans enzymes?

For Immunolocalization

01 September 2016 4,586 1 View

What is exactly the units of measure of the excitation signal provided by CST Microwave Studio? Is it Ampere or Volt or something else?

Is there any factor to consider for the excited signal? At the moment I am using a waveguide port. thank you.

19 April 2016 6,326 4 View

What is the best protocol for dissection and staining of the murine enteric nervous system?

I am trying to stain the dopaminergic neurons in the ENS of a mouse model for neurodegeneration. I need a protocol to isolate the ENS from the intestine and to prepare tissue sections for staining.

25 June 2015 2,346 5 View

How do anesthesiologists approach emergency {trauma} surgery when a patient has a full stomach and is also experiencing coughing?

Due to the motor vehicle accident or any other accident if a patient has already cough and cold and has full stomach then, How do anesthesiologists approach this type of emergency surgery ?

24 April 2024 9,897 3 View

Why do my orthotopic mouse models usually fail?

I have no idea why my orthotopic mouse models have failed. I have used gastric cancer cell lines, and I injected the cells into a mouse stomach. Here is my procedure and a result photo as a failed...

23 April 2024 1,923 9 View

How to extract the exosomes database?

Hello, what database should I read about exosomes and their relationship with cancer? Thank you for your answer And I wanted to do a thesis about exosomes related to stomach cancer. What is the...

28 December 2023 9,902 1 View

Hi, Anyone could help me for LMMP and whole mount protocol of rat stomach and intestine tissues?

For our LMMP protocol, first I placed the tissues in phosphate-buffered saline (PBS; 0.1 M, pH = 7.4) containing niphedipine for 15 min, then the tissues were fixated in 4% PFA in at 4 °C...

13 December 2023 2,925 0 View

What is the difference between Solea solea and Solea vulgaris from the taxonomical point of view?

What is the difference between the following species: Solea solea and Solea vulgaris from the taxonomical point of view?

28 November 2023 9,511 4 View

What is the significance of different dissolution media when performing a comparative dissolution kinetics test?

Hi all. I'm interested in a possibly trivial question. What is the real significance of pH 4.5 and 6.8 dissolution media when analyzing solid dosage forms when we have immediate release in pH 1.2...

02 November 2023 1,368 1 View

Are there any studies that measure cortisol levels during physical activity in short and long fasting states?

I would like to know how much cortisol increases in prolonged or brief states of fasting, to know how advisable physical activity on an empty stomach could be.

26 October 2023 9,640 0 View

What is a good percentage of release in the stomach to know if the material is a good oral delivery system directed to the small intestine?

Good morning. I am doing a drug release trial targeting the small intestine. I use a system where I change the release buffer at each sampling. So I get the cumulative percentage release. Two...

30 March 2023 8,163 0 View

How to isolate and identify H. pylori?

Hello everyone, I've been trying to isolate antibiotic resistant H. pylori from biopsy samples of stomach from dyspeptic UBT positive patients. The thing I am confused about is the exhibition of...

05 March 2023 536 0 View

What is the best in-vitro model for nitroso compound formation in stomach ? how to study if you developed formulation to inhibit the formation ?

Hi, global research concluded that nitroso compound formation in the stomach is the reason for gastric cancer. We developed a formulation for treatment. We would like to conduct an in-vitro model...

01 March 2023 8,531 0 View