How is it possible to obtain a more or less homogeneous solution containing cutin?
For enzyme assays using cell supernatant which are slight yellow and using pnpp substrate which will also produce the yellow pnp substrate will predictably have a distortion on the absorbance. to...
02 March 2024 5,149 3 View
I'm doing RNA dot blot with NC membrane. But its seems like something wrong with crosslinking. I'm wondering 1. If I need to use SSC buffer to treat with membrane before dotting? and what X of...
28 February 2024 4,705 1 View
can I use the cox activity assay kit with fish liver lyophilization samples??
13 December 2023 8,910 2 View
Hi, I am measuring the production of 4nitrophenol at 405nm for my enzyme assay. In the neg control (buffer + substrate) and the enzyme reaction at pH 5.5, 6.5 I observe the absorbance initially...
09 December 2023 1,091 4 View
I am wondering why there are two recipes to prepare 10X pbs, one for adjust ph value to 7.4, and the other one is to let the ph value remain around 6.8 after solving the salts. The latter one...
19 October 2023 2,882 0 View
first time asking question, trying to use UV light to excite Sn and SnO2 and detect the fluorescence, however there is nothing be detected. help
08 August 2023 4,537 4 View
Hello everyone; I am new to CRISPR. Owning to our strategy to screen positive cells, we yearn to involve two selection markers (GFP and puromycin) in the LentiCRISPRv2 vector. However, we figure...
18 July 2023 8,058 0 View
Surfactants with branched chains are known to reduce surface tension more than linear chain. However to what effect does a methyl branch on a propylene carbonate have compared to ethylene carbonate?
14 July 2023 1,678 0 View
I have a DSC result run from -90oC to 100oC in a heat/cool/heat cycle. Tg, crystallisation and melting peaks are observed in the heat run. However the cool run, the crystallisation peaks are not...
07 June 2023 2,747 3 View
While practicing the load cases in the publication "Design Load Basis for Offshore Wind Turbines - DT Wind Energy Report No. E-0133", a question came to me when I tried to simulate the DLC15...
30 May 2023 5,176 1 View
Hi How can this equation Ln(LA) = 1.038 + 0.89 ln(X) be applied to calculate the leaf area of a tomato? Can you explain with an example and what is the substitution of Ln and ln?
06 August 2024 2,508 2 View
I use 0.5% sarkosyl to solubilise recombinant proteins contained in Inclusion bodies of E. coli. sarkosyl very effeciently solubilizes proteins from Inclusion bodies and I am trying to use...
25 July 2024 5,001 2 View
I have conducted a study on the fructose, glucose, and sucrose compound content in tomato flesh extracts. The obtained peak areas aligned with the chromatogram graph. However, the concentrations...
19 July 2024 449 2 View
I'm using Dorsomorphin (C24H25N5O) as one component in dual SMAD inhibition of pluripotent stem cells to differentiate into neurons alongwith SB431542. Now as to solubilizing 10mg SB in DMSO for...
11 July 2024 4,832 0 View
I want to know the effect of KCl fertilizer application on chilli and tomato farm.
20 June 2024 3,486 2 View
In terms of labor, cost, and pollination efficiency, what is the most convenient way to pollinate tomatoes grown under controlled environment agriculture facilities, especially in greenhouses?...
18 June 2024 3,825 2 View
Hi everyone, there is my issue: I try to synthetize nanoparticule of Chitosan according with this study doi: 10.1016/j.colsurfb.2011.09.042 I try it with HMW Chitosan (340-360kDa) but when I put...
29 May 2024 1,763 2 View
Im extracting RNA from tomato leaves by the TRIZOL method but since the chloroform phase separation I can see that the aqueus phase is brownish and the pellet is totally brown. When I evaluate the...
21 May 2024 9,910 1 View
Hi, I am doing experiment of lignin deposition on tomato leaves after fungi infection by phloroglucinol-HCl staining. But the problem here is that the leaves remain yellowish or blackish before...
08 May 2024 2,127 0 View
I am purifying different proteins that go into inclusion bodies when expressed in E. coli. When I purify my inclusion bodies I always find two bands around 37 kDa in my solubilized fraction. These...
06 May 2024 6,324 2 View