Hello everyone,

I've been trying to isolate antibiotic resistant H. pylori from biopsy samples of stomach from dyspeptic UBT positive patients. The thing I am confused about is the exhibition of hemolytic activity by H. pylori on blood agar plates. After sub-culturing the small, translucent, non-hemolytic colonies that were urease positive, I proceeded for the 16srRNA sequencing identification. But the results of sequencing suggest the bacteria to be Lactobacillus paracasei which is normal flora of stomach. What could possibly be the best way to differentiate H. pylori from L. paracasei and isolate and identify it based on colony morphology, biochemical characteristics and hemolytic activity?

Looking forward to your responses