Which method of transfection will be more efficient and less time consuming, RELIABLE for stable transfection to try on endometrial cancer cell line ECC-1?
Time is very much limiting factor on my project, so I am looking for suggestion from experienced people who worked on both methods to choose the right one.
Lipofectamine 2000 or Electoporation?
Thanks.
Electroporation is (using the optimal protocol, e.g. see AMAXA settings) the most efficient method for cell transfection. However, this is mostly associated with a quite high death rate for the transfected cells. Lipofection is mostly much more gently but the transfection efficacy is often quite lower, compared to electroporation.
Yes, it may need time, if it is no standard protocol (e.g. from AMAXA) it may need some time, just as finding an adequate lipofection protocol.
- Badges
- Science method