Aruljothi Subramaniam

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Questions related from Aruljothi Subramaniam

Did anybody worked with EphA family protein expression? MW 130kDa . I am not getting any expression. Do you use different lysis buffer or RIPA?

I am working on EphA2, pEpA3 expression with different cell types (MD-MBA231, MOLT4) for the expression of EphA family proteins, They are of high molecular weight 130 kDa. I use RIPA buffer and...

02 December 2020 8,750 1 View

Any advice on best quality of orbital shaker or digital rotator on the market now for the membrane washing in Western Blotting?

Upgrading orbital shaker. Any advice on best quality of orbital shaker or digital rotator on the market now for the membrane washing in Western Blotting? Clean membrane washing is main concern...

17 December 2018 4,772 0 View

Do I need to use positive Control for all western blot - for transporter protein?

I am looking for transporter protein expression on cancer cell lines. Do I need to use transporter protein over-expressing cell line as positive control for my western blot experiment? for example...

01 November 2017 793 2 View

Normal cell line for comparison for liver cancer cell line

Can anyone recommend which normal cell line would be appropriate for comparison study for any drug treatment in compared with liver cancer cell lines?

20 July 2011 8,376 5 View

Which transfection method is more efficient and less time consuming, Lipofectamine 2000 ? Electroporation ? Why?

Which method of transfection will be more efficient and less time consuming, RELIABLE for stable transfection to try on endometrial cancer cell line ECC-1? Time is very much limiting factor on my...

01 January 1970 7,569 3 View