Hi, I am working on protein-protein interaction studies, specifically on antibody-antigen interaction. I would like to observe the changes in interaction if there's mutation occurs in the protein. Could anyone suggest a tool that can be used to induce substitution mutation to a targeted amino acid of a 3D protein and tools to validate that the mutation is not a nonsense mutation that produces truncated protein?
Hi, follow this one for part of your question:
swapaa (ucsf.edu)
You can use GUI of chimera to accomplish this.
Whether substitution truncate your protein, it can be simple be analyzed by translating the sequence, and look for the stop codon.
Hey,
You need to consider a few things:
Article Accurate proteome-wide missense variant effect prediction wi...
. AlphaMissense is specifically designed to predict the functional impact of missense mutations, offering a more targeted approach to understanding if these changes alter the function of the protein. They probably already tested your mutations, and you can find the score in the tables attached to the article.- Badges
- Science method