Which serum free media can I use for Cos-1 cells?

25 May 2015 1 8K Report

I would like to do cell uptake experiments using Cos-1 cells in serum free conditions. Which media can I use?

You can use complete DMEM as culture medium for COS-1 cells. For this you can please refer to the following link to get the complete composition of DMEM complete medium:-

http://link.springer.com/article/10.1007/BF02388316#page-2

Badges
Science method

More Goran Bajic's questions See All

How to edit preprint article in published article?

When I for the first time made available my the article Legal Aspects of Air Traffic Controller Liability, June 2022, it was in phase of the preprinting. After that when, status of the article...

10 December 2023 252 2 View

Indicator for detecting wastewater spills to surface water?

I'm working with a case where there is high risk of wastewater contamination to a lake that is raw water source for a treatment plant. Close to the raw water intake there are two wastewater pipes...

11 August 2022 6,280 3 View

How to provide reliability in P2P storage system?

I am familiar with a fact that in P2P storage distributed systems reliability is provided with redundancy using erasure code or simple file replication. I am interested if there are some other...

05 February 2021 1,699 3 View

How do forests influence avalanches and how protecting of the avalanches?

How do forests influence avalanches and how protecting of the avalanches? Forests play a very important protective role related to the avalanches. They act as a safeguard for communities and...

26 December 2020 9,514 1 View

How to Collect data from facebook?

I want to collect data from facebook group, data about users (when they are become member of group), interaction between members (comments and likes) etc. Question is what is best way to collect...

05 November 2020 6,570 6 View

Can anyone help interpret this circle shaped features found in iron-oxide nodule from the Pleistocene paleosol?

Red paleosol (RP) covering the carbonate basement exposed at the bottom of the loess section was investigated to give a systematic and deeper palaeopedological insight into reddish materials on...

21 August 2020 788 4 View

Does someone know which tubes can be used that are resistant to damage induced by homogenization (in a Bioruptor machine) in organic solvents?

My group is having problems with tubes that are damaged by homogenization in a Bioruptor because of the action of organic solvent (butyl acetate) exacerbated by the mechanical force. Our tubes are...

07 January 2020 5,927 0 View

What is best approach to model inter arrival time?

I am looking for best approach for modeling inter arrival times for series of events. I have dataset that present user file creation pattern in a period of one year. I model this number of files...

25 November 2019 4,081 0 View

How to track users accessing desktop file system?

I want to log user activity in a way to determine how they access their files on daily basis. For example, student have book in pdf and he read this file and other files during learning process....

04 October 2019 1,300 2 View

I am interested in papers that deal with land use changes / methodologies of using satellite images in different time series?

I am interested in papers that deal with land use changes / methodologies of using satellite images in different time series? I am working on some analyses of the impact of land use changes on...

06 August 2019 1,162 7 View

Which Scopus Journal provides the most affordable fees?

"PUBLISHING IN A SCOPUS JOURNAL" Researchers are now at a cross road. The critical need to publish in a Scopus or ISI, etc journal is ever vital. Journal Publication fees must be submitted....

10 August 2024 8,621 1 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Could you please suggest methods to compare free protein and immobolized protein binding properties?

I have an antibody binding generic protein and I need to compare its activity in a free and immobolized form. I understand that there are a number of methods to determine Kd value of a free...

05 August 2024 5,311 0 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View