Is there any interaction equivalent of Cochran's Q test (like 2x3 design)?

I have data on the effectiveness of the three treatments: T1, T2 and T3 for each patient. Each variable is coded dichotomously - 0 = drug not working; 1 = drug is working. The patient could feel...

30 November 2020 1,146 14 View

Two pair of peaks of quinone/hydroquinone redox probe on GCe?

Hello, I recently stumbled upon a voltammogram of HQ/Q in 0.1M NaCl on glassy carbon electrode. I was surprised to see two pairs of peaks present on the graph. As far as I know it is a two...

29 June 2020 1,833 2 View

Non-equal variances t-test in SPSS - it's Welch or Cochran-Cox?

Can anyone help me answer the question of whether t test for unequal variances is calculated in SPSS according to Welch or Cochran-Cox correction? Test statistics are the same, they differ only...

29 May 2019 7,445 14 View

Is there any reason why one can not reduce dimensions with PCA/Alpha before putting them into SEM model?

Suppose I have three measurable variables (A, B, C on figure below) and one latent (L) in my SEM model. This one latent one is a construct/dimension consisting of 5 questions from some...

07 April 2019 905 6 View

What is precipitated in buffer solution stored in glass bottle?

Does anyone know what is precipitated in carbonate buffer solution stored in typical amber glass bottle (picture is an attachment)? The carbonate buffer in the bottle is Na2CO3/NaHCO3, pH=10.0...

06 February 2019 7,556 3 View

How many animals are required for experiment?

I want to compare 4 different methods of bladder cancer induction and I have to calculate sample size. How many animals I have to used to get appropriate statistical power?

25 November 2018 151 2 View

How to generate average contact maps from molecular dynamics?

Hi, I am looking for a way to generate a contact map between each residue of a protein during MD simulations. I.e. for each pair of residues, count the number of frames during which they form...

05 May 2018 2,023 8 View

How to get rid of a primer smear in pcr (negative control problem)?

Hello everyone My pcr was doing just fine for few months till about 3 weeks ago I started to get smears in all my samples and positive and negative controls as well. I'm preparing my reaction...

21 March 2018 2,405 7 View

How can I cryopreservate Human Hepatic Stellate Cells?

Dear all, Does anyone know or have an experience in freezing (cryopreservation) of the primary Human Hepatic Stellate Cells? How much DMSO is recommended to use? I read that cryopreservation of...

03 March 2018 9,712 2 View

Freezing atoms in gaussian - how to do it?

Hello, I'm working on a structure with about 200 atoms. It is important for me to freeze a significant part of it for the time of optimization so that the arrangement of the given atoms with each...

05 February 2018 649 7 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View