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Questions related from Ananda Ayyappan Jaguva Vasudevan
If I have an antibody enriched in a sample and I would like to know which antigen to employ, what techniques can I use? Do you think methods such as co-IP coupled with mass spec analysis is the...
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Why do some protocol uses CaCl2 in the lysis and elution buffers while purifying the soluble protein from E.coli?
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Why do we need BSA as a blocking buffer for certain antibodies? What is different from casein? Thanks, Anand
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