Can anyone help me to find the complete sequence for plasmid pRG970?

I am trying to find out the full length sequence for above mentioned plasmid. I could not find in ncbi. Can anyone please tell me which site I should use? I will be grateful for any valuable...

01 February 2016 9,906 1 View

How can I limit the Large variation of dual luciferase assay (Promega) ratio between dublicate samples?

08 September 2015 6,146 18 View

How can I get a good signal of Myc associated factor X (MAX) protein in Western blot?

I have been trying to overexpress c-Myc and MAX in HEK 293 cell either alone or by co-transfection. c-Myc is over-expressed with high signal. However MAX has very low signal. I tried to check the...

06 July 2015 6,852 2 View

What is the best way for overexpression of Myc associated factor X (MAX)?

I am trying to overexpress human c-myc and MAX protein on HEK293 cells? I have no problem with c-myc but I detect negligible amount of MAX. When i perform the co-transfection, initial growth of...

06 July 2015 9,870 3 View

Can we lyse cells over expressing c-Myc Protein with 8M urea lysis buffer by gentle rotating overnight at room temperature?

I am purifying c-Myc partial protein after overexpression in Rosetta cells. 8 M urea precipitates at lower temperatures so I attempted to lyse the Rosetta cells overnight at room temperature by...

05 June 2015 4,557 5 View

Elisa IgG isotypes quantification problem?

I have posted my latest part of Elisa quantification result. Just the nature of O.D of the standard seems to affect the result a lot. Even if the O.D seems quite similar among different isotypes...

07 August 2014 1,334 10 View

I have been attempting to quantify virus specific IgG isotypes - any suggestions?

The problem I am encountering is, whenever I dilute the standard three fold I always find the absorbance of the first two wells almost similar despite the fact that the second well contains less...

05 June 2014 1,960 9 View

Can anyone suggest a way to get me out of an elution related problem during IgM purification?

I have been using the Thermo Scientific, Rockford, USA kit for IgM purification. During elution I don't feel like enough IgM is being eluted and its really troubling me. Any advice shall be highly...

03 April 2014 6,412 4 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

No title

02 March 2021 3,060 3 View