Which human cancer cell lines overexpress c-Myc constitutively?

09 October 2015 9,009 4 View

How can I limit the Large variation of dual luciferase assay (Promega) ratio between dublicate samples?

08 September 2015 6,146 18 View

How can I get a good signal of Myc associated factor X (MAX) protein in Western blot?

I have been trying to overexpress c-Myc and MAX in HEK 293 cell either alone or by co-transfection. c-Myc is over-expressed with high signal. However MAX has very low signal. I tried to check the...

06 July 2015 6,852 2 View

What is the best way for overexpression of Myc associated factor X (MAX)?

I am trying to overexpress human c-myc and MAX protein on HEK293 cells? I have no problem with c-myc but I detect negligible amount of MAX. When i perform the co-transfection, initial growth of...

06 July 2015 9,870 3 View

Can we lyse cells over expressing c-Myc Protein with 8M urea lysis buffer by gentle rotating overnight at room temperature?

I am purifying c-Myc partial protein after overexpression in Rosetta cells. 8 M urea precipitates at lower temperatures so I attempted to lyse the Rosetta cells overnight at room temperature by...

05 June 2015 4,557 5 View

Elisa IgG isotypes quantification problem?

I have posted my latest part of Elisa quantification result. Just the nature of O.D of the standard seems to affect the result a lot. Even if the O.D seems quite similar among different isotypes...

07 August 2014 1,334 10 View

I have been attempting to quantify virus specific IgG isotypes - any suggestions?

The problem I am encountering is, whenever I dilute the standard three fold I always find the absorbance of the first two wells almost similar despite the fact that the second well contains less...

05 June 2014 1,960 9 View

Can anyone suggest a way to get me out of an elution related problem during IgM purification?

I have been using the Thermo Scientific, Rockford, USA kit for IgM purification. During elution I don't feel like enough IgM is being eluted and its really troubling me. Any advice shall be highly...

03 April 2014 6,412 4 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View