Avishekh Gautam

9 Questions 24 Answers 0 Followers

Questions related from Avishekh Gautam

Can anyone help me to find the complete sequence for plasmid pRG970?

I am trying to find out the full length sequence for above mentioned plasmid. I could not find in ncbi. Can anyone please tell me which site I should use? I will be grateful for any valuable...

02 February 2016 9,941 1 View

Which human cancer cell lines overexpress c-Myc constitutively?

I am working with c-Myc and cancer. Which cancer cell lines over-express c-Myc? I would be grateful for suggestions.

10 October 2015 9,035 4 View

How can I limit the Large variation of dual luciferase assay (Promega) ratio between dublicate samples?

I am carrying out the dual luciferase Assay (Promega) on different cell lines. I am a beginner and I find abnormally huge variations of the dual luciferase assay ratios between duplicate samples...

09 September 2015 6,162 18 View

How can I get a good signal of Myc associated factor X (MAX) protein in Western blot?

I have been trying to overexpress c-Myc and MAX in HEK 293 cell either alone or by co-transfection. c-Myc is over-expressed with high signal. However MAX has very low signal. I tried to check the...

07 July 2015 6,881 2 View

What is the best way for overexpression of Myc associated factor X (MAX)?

I am trying to overexpress human c-myc and MAX protein on HEK293 cells? I have no problem with c-myc but I detect negligible amount of MAX. When i perform the co-transfection, initial growth of...

07 July 2015 9,894 3 View

Can we lyse cells over expressing c-Myc Protein with 8M urea lysis buffer by gentle rotating overnight at room temperature?

I am purifying c-Myc partial protein after overexpression in Rosetta cells. 8 M urea precipitates at lower temperatures so I attempted to lyse the Rosetta cells overnight at room temperature by...

06 June 2015 4,585 5 View

Elisa IgG isotypes quantification problem?

I have posted my latest part of Elisa quantification result. Just the nature of O.D of the standard seems to affect the result a lot. Even if the O.D seems quite similar among different isotypes...

08 August 2014 1,357 10 View

I have been attempting to quantify virus specific IgG isotypes - any suggestions?

The problem I am encountering is, whenever I dilute the standard three fold I always find the absorbance of the first two wells almost similar despite the fact that the second well contains less...

06 June 2014 1,987 9 View

Can anyone suggest a way to get me out of an elution related problem during IgM purification?

I have been using the Thermo Scientific, Rockford, USA kit for IgM purification. During elution I don't feel like enough IgM is being eluted and its really troubling me. Any advice shall be highly...

04 April 2014 6,439 4 View