Can anyone help me to find the complete sequence for plasmid pRG970?

I am trying to find out the full length sequence for above mentioned plasmid. I could not find in ncbi. Can anyone please tell me which site I should use? I will be grateful for any valuable...

01 February 2016 9,906 1 View

Which human cancer cell lines overexpress c-Myc constitutively?

09 October 2015 9,009 4 View

How can I limit the Large variation of dual luciferase assay (Promega) ratio between dublicate samples?

08 September 2015 6,146 18 View

How can I get a good signal of Myc associated factor X (MAX) protein in Western blot?

I have been trying to overexpress c-Myc and MAX in HEK 293 cell either alone or by co-transfection. c-Myc is over-expressed with high signal. However MAX has very low signal. I tried to check the...

06 July 2015 6,852 2 View

What is the best way for overexpression of Myc associated factor X (MAX)?

I am trying to overexpress human c-myc and MAX protein on HEK293 cells? I have no problem with c-myc but I detect negligible amount of MAX. When i perform the co-transfection, initial growth of...

06 July 2015 9,870 3 View

Can we lyse cells over expressing c-Myc Protein with 8M urea lysis buffer by gentle rotating overnight at room temperature?

I am purifying c-Myc partial protein after overexpression in Rosetta cells. 8 M urea precipitates at lower temperatures so I attempted to lyse the Rosetta cells overnight at room temperature by...

05 June 2015 4,557 5 View

Elisa IgG isotypes quantification problem?

I have posted my latest part of Elisa quantification result. Just the nature of O.D of the standard seems to affect the result a lot. Even if the O.D seems quite similar among different isotypes...

07 August 2014 1,334 10 View

I have been attempting to quantify virus specific IgG isotypes - any suggestions?

The problem I am encountering is, whenever I dilute the standard three fold I always find the absorbance of the first two wells almost similar despite the fact that the second well contains less...

05 June 2014 1,960 9 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

24 February 2021 1,243 1 View

How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View

Looking for advices and tips to improve the staining and the gating strategy in human blood?

Hi, I am staining a human blood with CD19, CD3,CD14 and CD16. My gating strategy in FlowJo goes like this: - First, I gate a general population SSC-A VS FSC-A => Then gate for single cells, =>...

21 February 2021 5,724 4 View

Does anyone have a working Guanidine Hydrochloride purification protocol for synthesized ssRNA?

I am trying to in vitro transcribe some fluorescein labeled ssRNA. And I want to purify the ssRNA from the free NTPs and the enzymes (RNA polymerase and DNase I). Due to the characteristic of the...

18 February 2021 4,392 4 View

Purification of proteins from baculovirus-infected insect cells?

18 February 2021 6,665 6 View

Agarose gel purification pre MinION sequencing?

Dear all, we performed an agarose gel purification with MinElute kit (QIAGEN) to extract bands between 3500 bp and 6000 bp for further sequencing with MinION. After the purification process,...

16 February 2021 5,178 1 View

Why samples do not run in resolving gel?

Hello everybody, I have a problem with SDS-PAGE and I do not know why my samples stop at the top of the resolving gel and they do not run through it. The percentage of gel is 15% and the size of...

14 February 2021 9,116 6 View