Which blocking buffer for DNA detection for ELISA?

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I am desperately trying to find a suitable stats analysis to test if my sightings confirm that terrapins prefer still or flowing water, please help?

I completed a survey and gained 137 sightings of terrapins, 87 in still water and 47 in flowing water. I originally thought I could do Chi goodness of fit but a stats book I have says I cant do...

24 April 2021 9,671 8 View

I've mistakenly put the cell that i wanted to freeze in the Room temperature overnight with 10%DMSO & 90%FBS. I wonder if the cell can still be alive?

27 May 2019 5,343 1 View

How to uncoagulate /ungel plasma extract sample for HPLC?

I have prepared multiple samples in the past just fine. Today, three of my 7 samples (all prepared from same plasma) coagulated. Is there a way to uncoagulate them? Is there a way to prevent this...

31 January 2019 7,348 0 View

Recommended programs to systematically identify genes within some distance of a response element in the genome?

I'm trying to find any programs or published scripts that can be used to systematically identify and return a list of genes within a specified distance of a response element. I have a list of...

08 January 2019 2,694 3 View

What questions would academics like to be able to answer using ParaSports Data data?

Is there data that people would like to see included that we can seek out that would be useful for academics conducting research related to disability and disability sport? The following are...

13 November 2018 7,441 1 View

Can you suggest any vocabulary size assessment tools to guide designing speaking tasks?

We are looking for a standardized test to measure students' vocabulary size to guide designing speaking tasks. The speaking tasks will be designed in line with CEFR and Pearson GSE topics. The...

18 September 2017 9,054 3 View

Poll: Do YOU think there is a Prime Equation?

An equation that states "This is the first Prime; this is the second..." etc, and keep this in mind, the probability for finding the NEXT prime among X is: Pˉ¹(x) ~ x / log(x) This is...

25 May 2017 8,852 7 View

How to apply the IAST for CO2/CH4 step by step?

I would like to calculate the selectivity of CO2/CH4 using IAST however I have not come across any paper explaining anything step by step. I have all the formulas required but I dont know how to...

25 January 2017 8,382 4 View

SDS-PAGE: Two gels run simultaneously proceed at different speeds?

We've had a problem in my lab where running two SDS-PAGE gels at once has resulted in the proteins/dye in one gel migrating much faster than the other, so that we have to pull out one gel and...

21 July 2016 9,709 8 View

How to get the Image number info in DICOM files?

How to get the Image number info in DICOM files? I tried 'dicominfo' but it doesn't get the 'image number' info in the DICOM header.

13 February 2016 7,587 9 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Hello researchers Is this a random laser or just fluorescence?

I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.

09 August 2024 9,894 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View

AMAXA Lonza nucleofection lower volume of buffer?

Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?

07 August 2024 4,616 0 View

Why I can't see any band in SDS-PAGE?

Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...

06 August 2024 5,373 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Why might the impedance values for DI water and 0.1X PBS buffer solution exhibit a decreasing and increasing trend, respectively over time (HP 4194A)?

Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...

05 August 2024 3,783 2 View

Binder for ZnO nanoparticles in graphite paste electrode?

I have been using paraffin, but the deposited ZnO still detach from electrode. What is the best binder to modify graphite paste electrode with ZnO nanoparticles?

03 August 2024 4,624 3 View