Which blocking buffer for DNA detection for ELISA?

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I've mistakenly put the cell that i wanted to freeze in the Room temperature overnight with 10%DMSO & 90%FBS. I wonder if the cell can still be alive?

27 May 2019 5,246 1 View

Recommended programs to systematically identify genes within some distance of a response element in the genome?

I'm trying to find any programs or published scripts that can be used to systematically identify and return a list of genes within a specified distance of a response element. I have a list of...

08 January 2019 2,629 3 View

SDS-PAGE: Two gels run simultaneously proceed at different speeds?

We've had a problem in my lab where running two SDS-PAGE gels at once has resulted in the proteins/dye in one gel migrating much faster than the other, so that we have to pull out one gel and...

21 July 2016 9,575 8 View

Plasmid DNA vs cDNA for qPCR standard curve?

I will be performing qPCR with cDNA templates. Is it okay to use a plasmid containing my target oglio for my standard curve or do I need to use cDNA dilutions? What are the drawbacks and/or...

07 April 2014 4,610 7 View

Lentiviral vector resuspension and parameters of ultracentrifugation step?

We are producing Lentiviral Vectors with CaPO4 transfection. And trying to optimize our protocol. There are two steps that we couldn't decide. One of them is the speed and degree of...

12 February 2014 7,858 4 View

What do academics researching disability want from a disability information database?

ParaSports Data has a lot of data about disability, disability sports and to a smaller extent disability rights. We want the database to be usable by more academics as a tool to facilitate...

01 January 1970 3,467 2 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Can chitosan alone can act as a reducing agent for Cu(0) nanoparticle sythesis?

[Synthesizing Cu nanoparticles without using chemical reductants]

01 March 2021 8,359 1 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View