Alemu Tekewe Mogus

5 Questions 226 Answers 0 Followers

Questions related from Alemu Tekewe Mogus

Does the pre-induction OD600 affect expression of stable proteins from bacteria?

We often induce protein expression when OD600 of E. coli culture reaches 0.4-0.8. What is/are the effect(s) of the pre-induction OD600 on expression level and stability of target proteins from...

20 March 2018 1,071 2 View

I am able to detect DNA bands on agarose gel from negative controls of PCR products? Did any one comeacross the same problem?

Recently, I have performed PCR reactions to amplify different gene fragments for cloning. I usually included negative controls in my experiments. The negative controls contain all the reaction...

30 August 2017 9,468 9 View

Why do HIV vaccine candidates developed so far fail to show good protective efficacy during preclinical and/or clinical trials?

It has been more than three decades since HIV/AIDS has already spread throughout all the five continents (North America, South America, Europe, Africa, Asia and Australia). A lot has been done to...

18 July 2017 1,032 6 View

Does any one come across an E. coli strain(s) that are secreting recombinant proteins into the extracellular environment?

Simplified downstream processing techniques are required to reduce the overall cost of bioprocessing of biological products, such as vaccines, antibodies and other recombinant pharmaceutical...

30 June 2017 3,598 3 View

Is it possible to engineer E. coli strains that do not produce endotoxins?

E. coli strains are good working horses for expressions of recombinant proteins. They have got huge potential for production of low-cost subunit vaccine candidates against various target diseases....

19 June 2017 9,995 11 View