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Questions related from Alemu Tekewe Mogus
We often induce protein expression when OD600 of E. coli culture reaches 0.4-0.8. What is/are the effect(s) of the pre-induction OD600 on expression level and stability of target proteins from...
20 March 2018 1,087 2 View
Recently, I have performed PCR reactions to amplify different gene fragments for cloning. I usually included negative controls in my experiments. The negative controls contain all the reaction...
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I am wondering why do we often use 70% ethanol for antisepsis and disinfection. Does this mean 70% ethanol is more effective than 80%, 90% or absolute ethanol? I appreciate if some one is able to...
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It has been more than three decades since HIV/AIDS has already spread throughout all the five continents (North America, South America, Europe, Africa, Asia and Australia). A lot has been done to...
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Simplified downstream processing techniques are required to reduce the overall cost of bioprocessing of biological products, such as vaccines, antibodies and other recombinant pharmaceutical...
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E. coli strains are good working horses for expressions of recombinant proteins. They have got huge potential for production of low-cost subunit vaccine candidates against various target diseases....
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