How to read a flow cytometry graph detailing cell cycle?

Hello everyone, When determining cells in different stages of the cell cycle, cells are stain for Brdu and Propidium Iodide. I understand how PI can be used to discriminate between G1 and S/G2...

18 February 2021 3,920 3 View

Do you know a good FGFR4 antibody for PDX IHC?

Dear all, I've tried many FGFR4 antibodies I saw in the literature but some of them are not useful for IHC and some are only useful for patients slides not for PDX. Do you know a FGFR4 antibody...

14 February 2021 900 0 View

How to improve NHBE adhesion after subculture?

I'm trying to subculture NHBE cells from Lonza, however, the cells seem to not adhere to the new flasks after detachment. I've followed Lonza detachment protocol (2 mL/25 cm2 of Trypsin 0.05% and...

10 February 2021 196 1 View

How to use the bioluminescent ATP assay kit?

I need to do a mitochondrial ATP quantification assay in insects and for this, I have a bioluminescent ATP assay kit but I don't have a protocol yet. I'd like to know if you have some kind of...

24 January 2021 3,655 3 View

How to perform Potential Surface Energy Exploration (PES) calculations?

I would like to receive help, as I am trying to make an analysis of the ethanol PES, but the calculations are always giving error. I use the conda-forge version of xtb (* xtb version 6.3.2...

04 January 2021 602 2 View

What is the difference between Introduction, Background, Literature Review and Conclusion for writing a Thesis Report?

I've recently started writing a thesis report based on my research paper. And I'm very confused about writing the chapter of the literature review because we already discusse everything in the...

03 January 2021 6,028 8 View

Sophie Germain Prize?

26 December 2020 9,273 6 View

How do you transmit and evaluate ethics and moral values in you teaching activity?

Ethics and moral values have always being an essential part of teaching and scientific research. How do you teach and evaluate these essential subjects in your daily interaction with students?

23 December 2020 5,058 7 View

Can I use the images of Collection 1 Level 2 Landsat 5 TM / Landsat 8 OLI to perform vegetation indices right away?

I'm aware that in order to perform any vegetation index like NDVI, SAVI or OSAVI you need to do some atmospheric, radiometric and terrain corrections. I'm pretty sure that Level 2 images from...

21 December 2020 4,088 3 View

How to determine Forster radius of a FRET pair?

Dear all, I'm searching for methods to calculate Forster Radii of FRET pairs, ideally for water-soluble and membrane dyes. Although I am relatively familiar with working with microscopy FRET, I am...

07 December 2020 3,051 3 View

Does any one have experience with Autoclavable cell culture media (RPMI 1640/DMEM)? Does it work well?

12 February 2021 2,958 1 View

Moving bacteria-like organisms in my HEK293T cell culture?

Hi everyone, I have been culturing HEK293T cells that have been engineered to overexpress the mechanosensitive protein Piezo1. However, I am finding it very difficult to keep them in culture -...

02 February 2021 7,892 4 View

There are prominent crystal formation in my cell culture dish, what are the possible causes and how to get rid of them?

17 December 2020 3,093 4 View

Why are my ARPE-19 cells exhibiting this morphology?

Hello! I am culturing ARPE-19 cells (passage 25) in DMEM + Sodium Pyruvate, Glutamine, 1% FBS and observing this phenotype in my cultures. These have been in culture for about 2 months and are...

12 May 2020 8,499 1 View

Alternative Media for Expi293s (Currently Using F17)?

Does anyone know of a more affordable alternative to F17 media for 293Expis? We currently supplement with L-glutamine and Pluronic F-68.

10 February 2020 637 1 View

Why L-asparaginase enzyme could give low cytotoxicity results in vitro on an anticancer experiment?

I'm currently working on my graduation project on the anticancer potential of E. coli L-asparaginase enzyme on mcf-7 along with other chemotherapy drugs. Previous references did prove cytotoxic...

10 November 2019 2,447 3 View

SDS-PAGE of a 410-kDa protein with pI 10.44?

Dear colleagues, I am working on a protein with peculiar characteristics. It is reasonably abundant in stem and cancer cells: - Size: 410 kDa - pI: 10.44 - Unusually rich in isoleucine and...

18 September 2019 321 2 View

SDS-PAGE of a 410-kDa protein with pI 10.44?

Dear colleagues, I am working on a protein with peculiar characteristics. It is reasonably abundant in stem and cancer cells: - Size: 410 kDa - pI: 10.44 - Unusually rich in isoleucine and...

08 September 2019 7,759 2 View

Any recommended handling method for neuroblastoma SH-SY5Y cells?

Good afternoon, I have some question related to the handling method for neuroblastoma cells SH-SY5Y. First, I grew the cells with EMEM medium (10% FBS and 1% Penicillin/Streptomycin). The cells...

07 August 2019 8,334 9 View

Any suggestions for PBMC/monocyte culture conditions (I get cells to attach well after isolation but then they die the next day)?

I have tried isolating PBMCs (looking to culture monocytes and differentiate into macrophages) from human blood using Ficoll gradient. I get a good amount of cells that attach. After 2-3 hours I...

06 July 2019 714 6 View