Rafael Andres Garcia

3 Questions 9 Answers 0 Followers

Questions related from Rafael Andres Garcia

Which amino acid side chains does OPA (o-phthaldialdehyde) react with?

I know it reacts with the alpha-amino group, but I'm only interested in the side chains. I think it reacts with lysine, but what about glutamine or asparagine?

22 November 2017 5,293 3 View

How can I deamidate glutamine and asparagine residues in protein, but leave the protein otherwise intact?

I don't care if the protein conformation or biological activity is lost. I just want to change Asn and Gln to Asp and Glu. I do want to avoid proteolysis.

07 July 2017 9,247 3 View

Is it really necessary create a new calibration curve each day for spectrophotometric assays?

I have always been taught to do this, but without any explanation of the reason. Does the instrument response really vary that much from day to day, given that you allow the lamp adequate time to...

01 February 2016 1,284 12 View