Are there any good specialists on the packages "leaps" and "SpaMM" in the R environment for collaboration?

My colleagues and I have done work on the polymorphism of flower color in one of the higher plant groups. But, unfortunately, it was rejected due to incorrect statistical analysis. We are just...

10 December 2020 885 3 View

How nanocatalyst loss can be minimized and effectively immobilized onto glass surface following AOPs in wastewater treatment?

Actually I have faced a significant quantity of nanocatalyst loss during immobilization on glass surface. Though I have used ethanol(volatile) to spread the catalyst effectively towards the...

22 September 2020 4,551 8 View

Does anyone have a good protocol for Ki67 staining for free floating sections?

My antibody didn't work at all. I have mouse brains, 30 um cryosectioned, and tried different concentrations of tween, as well as both formamide and SSC treatment to no avail.

18 December 2016 6,751 3 View

Please help me identify this Diptera. This family Tephritidae?

Collected September 2, 2016 in Donetsk, Ukraine

10 September 2016 930 17 View

Is this DiI staining valid or specific?

I placed DiI crystals into the hilus of P10 mice horizontal sections (vibratome). I think the first image is from the CA3 region, and I can't tell if these are dendrites/axons or background,...

01 February 2016 2,766 17 View

What is the most accurate and affordable pipette company?

27 October 2015 7,879 3 View

Can anybody recommend a good text on proposal writing and literature synthesis?

I am looking for suggestions from experts-what they suggest and students-what worked for you?

05 June 2014 3,721 3 View

Plant organ preservation in RNAlater for next generation transcriptome sequencing?

Does anyone have an RNA preservation protocol that yielded good next generation transcriptome sequencing results from preservation of plant organs in RNAlater (or another preservative) under field...

11 March 2014 1,359 3 View

How to make calculations with taking H2SO4 into account as a solvent in Gaussian 09?

I'd like to calculate relative energies for some molecules with taking a solvent into account. Reaction takes place in sulfuric acid, which is not included in the list of defined solvents in...

15 October 2013 7,150 7 View

How can one activate resting B cells and how can you check if they were really activated?

Dear colleagues, We are going to stimulate human B lymphocytes isolated from PBMCs from blood of healthy subjects (negative selection with MyltenilBiotech magnetic beads). It should be a control...

24 May 2012 2,800 29 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

Antibody anti-IgM Bovin conjugated Biotin ?

Hi everybody, Can anyone please suggest me an antibody anti-IgM Bovin conjugated Biotin ? I would like to use it in LUMINEX with a streptavidin conjugated phycoerythrin reporter. Thanks in...

25 February 2021 9,659 2 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View