I have recently started working with the HepG2 cell line. I am growing them in a High Glucose medium, with L-Glutamine and without phenol red.
As I have seen many pictures of the HepG2 cells on Google images, my cells look a bit different in morphology. There are no individual cells, most of the cells are growing in clusters with each other. I have attached a photo from today (not yet so confluent).
Before doing my experiments, I was curious to know if they are under any kind of stress.
Thank you so much.
Hello Mohd Ahmad
From the image, it seems that your seeding density is too low. How do you passage these cells? Increase the seeding density. Split sub-confluent cultures (which are 70-80% confluent) at 1:3 to 1:6.
The cells take nearly 5-6 days to form a confluent monolayer. The growth of HepG2 cells mainly occurs in islands.
Hope this helps!
Best.
Hi Malcolm Nobre I used the trypsinization method to passage the cell and was seeding around 250,000 cells in one T75 flask. However last time I seeded more cells and I have the new photos here.
The second image looks pretty good, as I've seen. I have worked for a long time on HepG2 cells. Normally, these cells grow in clusters. Mohd Ahmad
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