Where can I get LN-308 cell line?

Hi! I am looking for a cancer cell line with endogenously high expression of GPR55. LN-308 seems to have the highest level of GPR55 among multiple cancer cell lines (according to Genevestigator;...

20 October 2020 3,004 3 View

How can I quantify bacteria viable cells in a non-sterilized organic compost?

I'm working with the enrichment of an organic compost with PGPR, but this compost is not sterilized. How could I quantify viable cells of Azospirillum brasilense, Bacillus subtilis, and...

01 April 2020 9,486 4 View

How to model near-stall compressor operation with RANS CFD?

Hello everyone. I am looking into near-stall behaviour of the multistage axial compressor using CFD with RANS method. What is the best practice to establish if the compressor is close to stall,...

09 March 2020 8,847 8 View

What iron species are precipitating after autoclaving ferric chloride water solution?

I made 10mM FeCl3 solution in MQ water. After autoclaving it (standard 15min procedure) solution turns orange-brown. Filtering using 0.22um filter does not remove the color (but some of it is...

24 September 2019 2,407 7 View

What is a typical RFU value for a "low concentration" standard sample when quantifying RNA with Quantus Fluorometer?

Hi, quick question, What typical RFU value should I expect for a "low concentration" standard sample when quantifying RNA with Quantus Fluorometer? I made two standards (100 uL dye buffer, 98 uL...

10 April 2018 5,090 3 View

RNAprotect reagent formulation?

Hello, For the project I am currently involved in, I am in need for a cheap, non-toxic, pH 7+, non-disruptive buffer for RNA stabilization of bacterial cells (applied to a large environmental...

04 March 2018 5,089 14 View

How much does ammonium sulfate precipitation affect nucleic acid integrity?

Hello, In my current project I am at present trying to tackle an issue of rapidly stabilizing bacterial RNA in a large environmental sample. Most known stabilization reagents are not applicable in...

04 March 2018 1,589 3 View

SUOMI-NPP VIIRS DNB (Day-Night Band) radiance data and Light Pollution Assessment

We are working with SUOMI-NPP VIIRS DNB (Day-Night Band) radiance data, which has units of W cm-2 sr-1. The case study area is a bird sanctuary and an inhabited territory that is also used for...

14 May 2017 7,715 2 View

Is there a relation between Hadamard product and standard matrix product?

05 July 2015 5,366 7 View

Can we get the sufficient number of intensity measurements for a complex vector reconstruction in quantum tomography?

In a paper arXiv:1312.0158 the authors proved that any complex vector (thus also the quantum state vector) can be uniquely determined (up to a global phase factor) by 4N-4 generic intensity...

22 June 2015 7,125 4 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View