Has anybody used Oligofectamine for co-transfection of siRNA and Plasmid DNA?

I am planning to co-transfect siRNA and Plasmid DNA and I have only Oligofectamine with me. Though Oligofectamine is recommended only for siRNA transfection I was wondering if Plasmid DNA can also...

03 April 2017 8,664 3 View

Etoposide solubility!!! It precipitated in DMSO solution, what should I do?

Etoposide is soluble in DMSO up to 50mM concentration. I Prepared the solution accordingly and stored in -20 C a year back, now when I checked my stocks I found the solution was clear and a pellet...

10 November 2014 7,596 4 View

How should I analyze metabolites from cell culture media using Mass Spectrometry?

I need to check whether the level of metabolites are altered in cell culture media during specific treatment condition because of altered uptake and secretions.

31 December 2013 934 12 View

I am getting too much of variation in repeating MTT assay, any suggestions?

I am over-expressing some proteins using vector constructs and checking the effects of various chemical treatments on over-expression.

07 August 2013 6,879 2 View

How can I estimate protein amount after MTT cell death proliferation assay?

Does the MTT solubilizer interfere with the protein estimation at 280 nm? Can I use bradford to assay the protein amount?

07 August 2013 4,836 0 View

What concentration of camptothecin is effective to kill epithelial (Hek293) cells ?

i have prepared camptothecin in DMSO.

06 July 2013 6,083 7 View

we are using Rep-PCR technique for fungal typing, i want to know that why there is only one primer for the amplification of BOX element ???

in other Rep- PCR technique we have set of primer for ERIC and Rep repeat elements, there is only a single primer BOXA1R for the amplification of BOX elements.

11 December 2011 4,580 0 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View