Where can I get drug resistant MEFs (DR4 MEFs) in India?

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Has anybody used Oligofectamine for co-transfection of siRNA and Plasmid DNA?

I am planning to co-transfect siRNA and Plasmid DNA and I have only Oligofectamine with me. Though Oligofectamine is recommended only for siRNA transfection I was wondering if Plasmid DNA can also...

03 April 2017 8,734 3 View

Etoposide solubility!!! It precipitated in DMSO solution, what should I do?

Etoposide is soluble in DMSO up to 50mM concentration. I Prepared the solution accordingly and stored in -20 C a year back, now when I checked my stocks I found the solution was clear and a pellet...

10 November 2014 7,662 4 View

How should I analyze metabolites from cell culture media using Mass Spectrometry?

I need to check whether the level of metabolites are altered in cell culture media during specific treatment condition because of altered uptake and secretions.

31 December 2013 1,063 12 View

I am getting too much of variation in repeating MTT assay, any suggestions?

I am over-expressing some proteins using vector constructs and checking the effects of various chemical treatments on over-expression.

07 August 2013 6,948 2 View

How can I estimate protein amount after MTT cell death proliferation assay?

Does the MTT solubilizer interfere with the protein estimation at 280 nm? Can I use bradford to assay the protein amount?

07 August 2013 4,920 0 View

What concentration of camptothecin is effective to kill epithelial (Hek293) cells ?

i have prepared camptothecin in DMSO.

06 July 2013 6,146 7 View

we are using Rep-PCR technique for fungal typing, i want to know that why there is only one primer for the amplification of BOX element ???

in other Rep- PCR technique we have set of primer for ERIC and Rep repeat elements, there is only a single primer BOXA1R for the amplification of BOX elements.

11 December 2011 4,674 0 View

What are the emerging trends and opportunities for STEM education and careers in the online space?

10 August 2024 507 0 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is this a facetotecta nauplius?

This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.

08 August 2024 3,746 4 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Arumugam Muthu

It may be useful for you, please contact:

: Dr Thangam Menon, Professor & Head, Department of Microbiology, Dr A.L. Mudaliar Post Graduate

Institute of Basic Medical Sciences, University of Madras, Taramani, Chennai 600 113, India

e-mail: [email protected].

Milton Roy

Dear Sir,

Thanks a lot. I will contact Dr, Menon asap.