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Questions related from Milton Roy
I am planning to co-transfect siRNA and Plasmid DNA and I have only Oligofectamine with me. Though Oligofectamine is recommended only for siRNA transfection I was wondering if Plasmid DNA can also...
04 April 2017 8,713 3 View
Can anybody provide the cells in running culture?
12 December 2014 401 2 View
Etoposide is soluble in DMSO up to 50mM concentration. I Prepared the solution accordingly and stored in -20 C a year back, now when I checked my stocks I found the solution was clear and a pellet...
11 November 2014 7,643 4 View
I need to check whether the level of metabolites are altered in cell culture media during specific treatment condition because of altered uptake and secretions.
01 January 2014 1,041 12 View
Does the MTT solubilizer interfere with the protein estimation at 280 nm? Can I use bradford to assay the protein amount?
08 August 2013 4,886 0 View
I am over-expressing some proteins using vector constructs and checking the effects of various chemical treatments on over-expression.
08 August 2013 6,925 2 View
i have prepared camptothecin in DMSO.
07 July 2013 6,124 7 View
in other Rep- PCR technique we have set of primer for ERIC and Rep repeat elements, there is only a single primer BOXA1R for the amplification of BOX elements.
12 December 2011 4,651 0 View
