Does the MTT solubilizer interfere with the protein estimation at 280 nm? Can I use bradford to assay the protein amount?
I am planning to co-transfect siRNA and Plasmid DNA and I have only Oligofectamine with me. Though Oligofectamine is recommended only for siRNA transfection I was wondering if Plasmid DNA can also...
03 April 2017 8,734 3 View
Can anybody provide the cells in running culture?
11 December 2014 426 2 View
Etoposide is soluble in DMSO up to 50mM concentration. I Prepared the solution accordingly and stored in -20 C a year back, now when I checked my stocks I found the solution was clear and a pellet...
10 November 2014 7,662 4 View
I need to check whether the level of metabolites are altered in cell culture media during specific treatment condition because of altered uptake and secretions.
31 December 2013 1,063 12 View
I am over-expressing some proteins using vector constructs and checking the effects of various chemical treatments on over-expression.
07 August 2013 6,948 2 View
i have prepared camptothecin in DMSO.
06 July 2013 6,146 7 View
in other Rep- PCR technique we have set of primer for ERIC and Rep repeat elements, there is only a single primer BOXA1R for the amplification of BOX elements.
11 December 2011 4,674 0 View
Hello, So I have mouse brain tissue MS data from one of my predecessors and I have human serum data from the hospital that I collaborate with. We are trying to find markers for the diagnosis of...
11 July 2024 8,484 0 View
I need some articles on the analysis of the nutritional quality of barley (H.vulgare.L) and the analytical approach of metabolomics and proteomics.
26 June 2024 3,451 2 View
Hi everyone, I wanna do proteomics of monkeys serums in 2 states, healthy and patient. How many monkeys in each group I need to run proteomics and validate my data from it? Do I deplete albumin...
04 June 2024 4,091 3 View
I have a proteomics dataset with missing values. I tried some strategies, but the point is that there are sets completely with missing values. The last strategy was to apply MissForest in python...
29 May 2024 5,563 4 View
We want to find interacting protein partners for our protein of interest.
28 April 2024 921 1 View
Due to factors outside of my control, peptides in my ESI-MS data have been ionised normally by protons ([M+2H], [M+3H]...) but also by sodium ([M+2Na], [M+3Na]...). Is there a way to configure...
16 April 2024 10,028 2 View
Hello, In the literature, there are some MS/MS results that include hypothetical proteins, which can be shorter than 40 amino acids. I can also find these when I search for an organism in the...
04 April 2024 5,098 2 View
How many ug/particles of EVs is needed for doing proteomics? We are doing many steps for purify EVs of human plasma and at the end of the process we are getting just 1/2ug/ml concentration of...
25 March 2024 730 1 View
serum proteomics
17 February 2024 1,086 3 View
Could this be due to an error in Mass Spec calibration or data analysis? I have 2 technical repeats that are fine, but the 3rd repeat is far away in the PCA plot and clusters with replicates of a...
05 February 2024 5,464 3 View