Does the MTT solubilizer interfere with the protein estimation at 280 nm? Can I use bradford to assay the protein amount?
I am planning to co-transfect siRNA and Plasmid DNA and I have only Oligofectamine with me. Though Oligofectamine is recommended only for siRNA transfection I was wondering if Plasmid DNA can also...
03 April 2017 8,664 3 View
Can anybody provide the cells in running culture?
11 December 2014 354 2 View
Etoposide is soluble in DMSO up to 50mM concentration. I Prepared the solution accordingly and stored in -20 C a year back, now when I checked my stocks I found the solution was clear and a pellet...
10 November 2014 7,596 4 View
I need to check whether the level of metabolites are altered in cell culture media during specific treatment condition because of altered uptake and secretions.
31 December 2013 934 12 View
I am over-expressing some proteins using vector constructs and checking the effects of various chemical treatments on over-expression.
07 August 2013 6,879 2 View
i have prepared camptothecin in DMSO.
06 July 2013 6,083 7 View
in other Rep- PCR technique we have set of primer for ERIC and Rep repeat elements, there is only a single primer BOXA1R for the amplification of BOX elements.
11 December 2011 4,580 0 View
Hi I am very new to proteomics analysis. I have got raw files from core facility running thermofisher TMT 16plex 4 hours. I cannot find on maxquant the option TMT16Plex there is only up to 11...
25 February 2021 1,675 3 View
Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...
23 February 2021 6,671 4 View
Hi, so basically... I am trying to mimic a clinically observed condition wherein a frame-shift mutation happens only at one allele of the patients, giving rise to a truncated variant which evades...
17 February 2021 1,130 6 View
Hello! I would like to know if there is a way to flush/remove all the cells out of a tissue and keep only the extracellular matrix, in order to analyse its composition using e.g. proteomics....
14 February 2021 1,602 4 View
Study bringing together genomics, proteomics etc together
02 February 2021 2,856 2 View
I planned an experimental setup using 10-plex TMT with 9 conditions and 1 bridge channel to compare 5 replicates. But since I experienced some run-to-run variation, I was wondering if a better...
31 January 2021 3,405 3 View
Hi, I'm working with NanoLC/MS for both Metabolomics and Proteomics. We usually make our nano columns using emitters from NewObjective. Unfortunately New Objective, due to the pandemic, is now...
10 January 2021 1,176 4 View
Hi everyone, I am starting a project in analyzing EVs proteomics in cancers. However, the most published articles I have found stated only brief isolation protocols that could not fully be...
17 December 2020 7,600 2 View
Hi, I am trying to screen infected plant proteomes for targets of a secreted pathogen protein. I have purified the pathogen protein using a GST-tag on an agarose resin. I am using the Pierce...
23 November 2020 7,009 3 View
Hi, I want the MS2-CID spectra from orbitrap velos mass spectrometry of organism: human (Mod: C+57) for testing my peptide identification algorithm developed. Large set of orbitrap velos dataset...
16 November 2020 8,261 1 View