Where can I get drug resistant MEFs (DR4 MEFs) in India?

Can anybody provide the cells in running culture?

11 December 2014 354 2 View

Etoposide solubility!!! It precipitated in DMSO solution, what should I do?

Etoposide is soluble in DMSO up to 50mM concentration. I Prepared the solution accordingly and stored in -20 C a year back, now when I checked my stocks I found the solution was clear and a pellet...

10 November 2014 7,596 4 View

How should I analyze metabolites from cell culture media using Mass Spectrometry?

I need to check whether the level of metabolites are altered in cell culture media during specific treatment condition because of altered uptake and secretions.

31 December 2013 934 12 View

I am getting too much of variation in repeating MTT assay, any suggestions?

I am over-expressing some proteins using vector constructs and checking the effects of various chemical treatments on over-expression.

07 August 2013 6,879 2 View

How can I estimate protein amount after MTT cell death proliferation assay?

Does the MTT solubilizer interfere with the protein estimation at 280 nm? Can I use bradford to assay the protein amount?

07 August 2013 4,836 0 View

What concentration of camptothecin is effective to kill epithelial (Hek293) cells ?

i have prepared camptothecin in DMSO.

06 July 2013 6,083 7 View

we are using Rep-PCR technique for fungal typing, i want to know that why there is only one primer for the amplification of BOX element ???

in other Rep- PCR technique we have set of primer for ERIC and Rep repeat elements, there is only a single primer BOXA1R for the amplification of BOX elements.

11 December 2011 4,580 0 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View